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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ195099:GJC0-1231-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CJE1204
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000436 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Isoleucine--tRNA ligasePRO_0000098373Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi195099.CJE1204.

Structurei

3D structure databases

ProteinModelPortaliQ5HU41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HU41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLLP STSFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK
60 70 80 90 100
SFTLHDGPPY ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG
110 120 130 140 150
LPIEQQVEVK LGEKKKSLSK KEIREFCRQH ASEFVDIQRE EFKNLGIIAD
160 170 180 190 200
WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC ERSKPVFWSW AAKSALAEAE
210 220 230 240 250
VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA STVIWTTTPW TLVANQAIAL
260 270 280 290 300
NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP
310 320 330 340 350
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD
360 370 380 390 400
SGCYDETLRA KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH
410 420 430 440 450
SYPFCWRTHK PVIYRATKQW FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ
460 470 480 490 500
SGVKRIGSMV ENRPDWCISR QRDWGTPIAF FRDKNTKEVI FDDELFDFVA
510 520 530 540 550
AIFEKHGADA WWEFEIKDLI PTNSKYNAEN LEKVYDILDV WFDSGSTFNA
560 570 580 590 600
VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH
610 620 630 640 650
GFTTDEKGQK MSKSKGNVIA PEYVAKTYGV EILRLWILLS DYLSDLKISD
660 670 680 690 700
NILKQVGEQY RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF
710 720 730 740 750
KASKEAFFAY EFAKGFSLLL NFLSADLSGI YLDISKDRLY CDSENAQRRK
760 770 780 790 800
SAQVAMALIA KELLNLLASN LSYSVDEALE HANVLIKGDA KDVFDLSLTQ
810 820 830 840 850
DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN LNISFNKFPN
860 870 880 890 900
EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKD
910
EETPCLRCEE VLKGIQC
Length:917
Mass (Da):105,286
Last modified:February 15, 2005 - v1
Checksum:i38D5B82C10E099D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1.
RefSeqiYP_179194.1. NC_003912.7.

Genome annotation databases

EnsemblBacteriaiAAW35529; AAW35529; CJE1204.
KEGGicjr:CJE1204.
PATRICi20044204. VBICamJej134361_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1.
RefSeqiYP_179194.1. NC_003912.7.

3D structure databases

ProteinModelPortaliQ5HU41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi195099.CJE1204.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW35529; AAW35529; CJE1204.
KEGGicjr:CJE1204.
PATRICi20044204. VBICamJej134361_1220.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCJEJ195099:GJC0-1231-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM1221.

Entry informationi

Entry nameiSYI_CAMJR
AccessioniPrimary (citable) accession number: Q5HU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.