Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei569Aminoacyl-adenylateUniRule annotation1
Binding sitei613ATPUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CJE1204
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983731 – 917Isoleucine--tRNA ligaseAdd BLAST917

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5HU41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi610 – 614"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HU41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLLP STSFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK
60 70 80 90 100
SFTLHDGPPY ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG
110 120 130 140 150
LPIEQQVEVK LGEKKKSLSK KEIREFCRQH ASEFVDIQRE EFKNLGIIAD
160 170 180 190 200
WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC ERSKPVFWSW AAKSALAEAE
210 220 230 240 250
VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA STVIWTTTPW TLVANQAIAL
260 270 280 290 300
NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP
310 320 330 340 350
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD
360 370 380 390 400
SGCYDETLRA KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH
410 420 430 440 450
SYPFCWRTHK PVIYRATKQW FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ
460 470 480 490 500
SGVKRIGSMV ENRPDWCISR QRDWGTPIAF FRDKNTKEVI FDDELFDFVA
510 520 530 540 550
AIFEKHGADA WWEFEIKDLI PTNSKYNAEN LEKVYDILDV WFDSGSTFNA
560 570 580 590 600
VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH
610 620 630 640 650
GFTTDEKGQK MSKSKGNVIA PEYVAKTYGV EILRLWILLS DYLSDLKISD
660 670 680 690 700
NILKQVGEQY RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF
710 720 730 740 750
KASKEAFFAY EFAKGFSLLL NFLSADLSGI YLDISKDRLY CDSENAQRRK
760 770 780 790 800
SAQVAMALIA KELLNLLASN LSYSVDEALE HANVLIKGDA KDVFDLSLTQ
810 820 830 840 850
DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN LNISFNKFPN
860 870 880 890 900
EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKD
910
EETPCLRCEE VLKGIQC
Length:917
Mass (Da):105,286
Last modified:February 15, 2005 - v1
Checksum:i38D5B82C10E099D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1.
RefSeqiWP_011049879.1. NC_003912.7.

Genome annotation databases

EnsemblBacteriaiAAW35529; AAW35529; CJE1204.
KEGGicjr:CJE1204.
PATRICi20044204. VBICamJej134361_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1.
RefSeqiWP_011049879.1. NC_003912.7.

3D structure databases

ProteinModelPortaliQ5HU41.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW35529; AAW35529; CJE1204.
KEGGicjr:CJE1204.
PATRICi20044204. VBICamJej134361_1220.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_CAMJR
AccessioniPrimary (citable) accession number: Q5HU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.