SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5HU41

- SYI_CAMJR

UniProt

Q5HU41 - SYI_CAMJR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, CJE1204
Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ195099:GJC0-1231-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CJE1204
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000436: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098373Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi195099.CJE1204.

Structurei

3D structure databases

ProteinModelPortaliQ5HU41.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HU41-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKETLLLP STSFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK    50
SFTLHDGPPY ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG 100
LPIEQQVEVK LGEKKKSLSK KEIREFCRQH ASEFVDIQRE EFKNLGIIAD 150
WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC ERSKPVFWSW AAKSALAEAE 200
VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA STVIWTTTPW TLVANQAIAL 250
NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP 300
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD 350
SGCYDETLRA KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH 400
SYPFCWRTHK PVIYRATKQW FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ 450
SGVKRIGSMV ENRPDWCISR QRDWGTPIAF FRDKNTKEVI FDDELFDFVA 500
AIFEKHGADA WWEFEIKDLI PTNSKYNAEN LEKVYDILDV WFDSGSTFNA 550
VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH 600
GFTTDEKGQK MSKSKGNVIA PEYVAKTYGV EILRLWILLS DYLSDLKISD 650
NILKQVGEQY RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF 700
KASKEAFFAY EFAKGFSLLL NFLSADLSGI YLDISKDRLY CDSENAQRRK 750
SAQVAMALIA KELLNLLASN LSYSVDEALE HANVLIKGDA KDVFDLSLTQ 800
DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN LNISFNKFPN 850
EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKD 900
EETPCLRCEE VLKGIQC 917
Length:917
Mass (Da):105,286
Last modified:February 15, 2005 - v1
Checksum:i38D5B82C10E099D4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1.
RefSeqiYP_179194.1. NC_003912.7.

Genome annotation databases

EnsemblBacteriaiAAW35529; AAW35529; CJE1204.
GeneIDi3231713.
KEGGicjr:CJE1204.
PATRICi20044204. VBICamJej134361_1220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000025 Genomic DNA. Translation: AAW35529.1 .
RefSeqi YP_179194.1. NC_003912.7.

3D structure databases

ProteinModelPortali Q5HU41.
ModBasei Search...

Protein-protein interaction databases

STRINGi 195099.CJE1204.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW35529 ; AAW35529 ; CJE1204 .
GeneIDi 3231713.
KEGGi cjr:CJE1204.
PATRICi 20044204. VBICamJej134361_1220.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CJEJ195099:GJC0-1231-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM1221.

Entry informationi

Entry nameiSYI_CAMJR
AccessioniPrimary (citable) accession number: Q5HU41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi