Reviewed, UniProtKB/Swiss-Prot Q5HTL1 (PUR2_CAMJR)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	CJE1387

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151440
Regions
	Domain	105 – 310	206	ATP-grasp
	Nucleotide binding	131 – 192	62	ATP By similarity
Sites
	Metal binding	281	1	Magnesium or manganese By similarity
	Metal binding	283	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HTL1-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: E700C38A97AFE9D6	FASTA	416	45,316
	10 20 30 40 50 60 MKIMILGSGA REYSIALALR RVDKNLEFYF APGNGATESL GTNLNLKDPV VLATYAKEKG 70 80 90 100 110 120 FDLCIVGSES FLAEGVVDIF KQQGLAIFGP SKAAAMLETS KSFMKSFLKK YRIKTAKFLN 130 140 150 160 170 180 TNDIEKAKNF IYSLTPPIVV KADGLCAGKG VIIAKTHEEA IEETAKMLSG ESFGDAGKLV 190 200 210 220 230 240 VIEEFLDGYE LSIFAVCDGN DFVLLPAAQD HKKLLDNDQG PNTGGMGAYA PSSLANESLL 250 260 270 280 290 300 RKVQKDIILP TLAGMKKEGA EFCGVLFIGA MIVGNKPYVL EFNVRFGDPE CEVLMPLIED 310 320 330 340 350 360 PLELILAATQ RRLRHSKIKI KKEFAVGVVC ASENYPYKSS PKSEITVNNI PENSHISYAG 370 380 390 400 410 VSLEDGKLMA DGGRVLVCVG TGKSIEEAQK NAYKLCDNVN FKGKQYRKDI AHQVLK

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000025 Genomic DNA. Translation: AAW35707.1.
RefSeq	YP_179374.1.
3D structure databases
SMR	Q5HTL1. Positions 1-411.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HTL1.
Genome annotation databases
GeneID	3231893.
GenomeReviews	Gene locus CJE1387 in contig CP000025_GR.
KEGG	cjr:CJE1387.
TIGR	CJE1387.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_1387-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_CAMJR

Accession

Primary (citable) accession number: Q5HTL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)