Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi507MagnesiumUniRule annotation1
Metal bindingi513MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • mRNA catabolic process Source: TIGR
  • RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:CJE1390
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003295721 – 719Polyribonucleotide nucleotidyltransferaseAdd BLAST719

Structurei

3D structure databases

ProteinModelPortaliQ5HTK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini573 – 633KHUniRule annotationAdd BLAST61
Domaini658 – 719S1 motifUniRule annotationAdd BLAST62

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 2 hits.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HTK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYSIEINKN TEIFNIDKVA KQAAGAVLMR QGKSVVLATV AREEKQVEED
60 70 80 90 100
FLPLTVQYIE KAYAAGKIPG GYVKRETKPS DAETLTARII DRSLRPLFPK
110 120 130 140 150
GYAYPTQIVV MVLSADPKVD LQVMSLNAAS VALYLSDIPM KAPVCGVRIG
160 170 180 190 200
KIDGNFILNP NNEELQNSTL DLYVAGVKDE LLMIEMRALP DQKENEIFIE
210 220 230 240 250
APYADVLTQT TSQNMNELSE DEILEALNLA QKAILNGSNA YEEAFSKHKK
260 270 280 290 300
NSQIELKNEI EYPEILAFIE NNFQKQIKEA INQMAKSERA SELNKIAKEI
310 320 330 340 350
SNLEIAKEWS EESVLNTLAK VKRKLIRGQI LNEGKRADGR SLNEVRPISI
360 370 380 390 400
ETNILPNAHG SCLFTRGQTQ ALVVATLGGE NDAQMIDLLT EKNPISERFM
410 420 430 440 450
VNYNFPGFSV GEASPIKAPG RRELGHGNLA KRALYPSVDE NYPYVIRLVS
460 470 480 490 500
EILESNGSSS MATVCGGSLA LKAAGVPSLK LVAGVAMGLI FEDNKYAVLT
510 520 530 540 550
DIMGLEDHDG DMDFKVAGSK DGVTALQMDI KLGGIDQETL KQALYQAKEG
560 570 580 590 600
RIHILNIMEE AAKEIIVNEE VLPKLELFSV DPSKIVDIIG QAGKTIKEIV
610 620 630 640 650
EKFGVSIDLD REKGEVKIAG SQNEQIKAAK DYIINITSSQ KGTKKGSKDK
660 670 680 690 700
DISGFELGQE FQGIVKKIAP FGAFVELKNG VDGLLHSSKS KHLNLSENQS
710
LKVKISEIKN GKISVDLCE
Length:719
Mass (Da):79,018
Last modified:February 15, 2005 - v1
Checksum:iE9EA95891AAE6FEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35710.1.
RefSeqiWP_002908583.1. NC_003912.7.

Genome annotation databases

EnsemblBacteriaiAAW35710; AAW35710; CJE1390.
KEGGicjr:CJE1390.
PATRICi20044580. VBICamJej134361_1407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000025 Genomic DNA. Translation: AAW35710.1.
RefSeqiWP_002908583.1. NC_003912.7.

3D structure databases

ProteinModelPortaliQ5HTK8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW35710; AAW35710; CJE1390.
KEGGicjr:CJE1390.
PATRICi20044580. VBICamJej134361_1407.

Phylogenomic databases

HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 2 hits.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_CAMJR
AccessioniPrimary (citable) accession number: Q5HTK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.