Q5HT36 (NTPA_CAMJR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

CJE1566

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178146
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	73 – 74	2	Substrate binding By similarity
Sites
	Metal binding	38	1	Magnesium or manganese By similarity
	Metal binding	73	1	Magnesium or manganese By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HT36 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 1E82869A8EAC8BA1	FASTA	200	22,349
	10 20 30 40 50 60 MKIILATSNK HKVLELKEIL KDFEIYAFDE VLMPFEIEEN GKTFKENALI KARAVFNALD 70 80 90 100 110 120 EKQKKDFIAL SDDSGICVDV LEGNPGIYSA RFSGKGDDKS NRDKLVNEMI KKGFKQSKAH 130 140 150 160 170 180 YVAAIAMVGL MGEFSTHGTM HGKVIDTEKG ENGFGYDSLF IPKGFDKTLA QLSVDEKNNI 190 200 SHRFKALELA KIILKILNKG

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM1221.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000025 Genomic DNA. Translation: AAW36001.1.
RefSeq	YP_179549.1. NC_003912.7.
3D structure databases
ProteinModelPortal	Q5HT36.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HT36.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3232194.
GenomeReviews	Gene locus CJE1566 in contig CP000025_GR.
KEGG	cjr:CJE1566.
PATRIC	20044924. VBICamJej134361_1576.
TIGR	CJE1566.
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	GVYTADW.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_1566-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CAMJR

Accession

Primary (citable) accession number: Q5HT36

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program