Reviewed, UniProtKB/Swiss-Prot Q5HSB7 (MURB_CAMJR)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CJE1848

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable HAMAP MF_00037.
Sequence similarities	Belongs to the murB family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179191
Sites
	Active site	142	1	By similarity
	Active site	184	1	Proton donor By similarity
	Active site	254	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HSB7-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: B3AE614135ED20EE	FASTA	258	29,041
	10 20 30 40 50 60 MIIDFKKYSS VRIGNEFEVL VLDQICDFDG FLIGGANNLL ISPKPKNIGI LGDGFDFMQI 70 80 90 100 110 120 LDQNKDFIHL RIGCKTKSSK IYRFAKENNL KGFEYLSKIP GTLGGLLKMN AGLKGECISQ 130 140 150 160 170 180 NLIKIATSQG EILRENINFD YRFCPLNMPF FWAEFKLNFG FDTLKDEALK NARSNQPSGA 190 200 210 220 230 240 SFGSIFKNPK NDFAGRLIEA VGLKGFSKGD AMLSDKHANF LINKKNASFE DAFFLIELAR 250 KKVFEEFGIN LENEVIII

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000025 Genomic DNA. Translation: AAW36270.1.
RefSeq	YP_179818.1.
3D structure databases
SMR	Q5HSB7. Positions 5-258.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HSB7.
Genome annotation databases
GeneID	3232475.
GenomeReviews	Gene locus CJE1848 in contig CP000025_GR.
KEGG	cjr:CJE1848.
NMPDR	fig|195099.3.peg.1789.
TIGR	CJE1848.
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	LVKMNAG.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_1848-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR003170. MurB. IPR011601. MurB_C. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURB_CAMJR

Accession

Primary (citable) accession number: Q5HSB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)