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Q5HSB2

- BIOB_CAMJR

UniProt

Q5HSB2 - BIOB_CAMJR

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Protein
Biotin synthase
Gene
bioB, CJE1853
Organism
Campylobacter jejuni (strain RM1221)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi20 – 201Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi23 – 231Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi95 – 951Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi153 – 1531Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCJEJ195099:GJC0-1892-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:CJE1853
OrganismiCampylobacter jejuni (strain RM1221)
Taxonomic identifieri195099 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000436: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Biotin synthaseUniRule annotation
PRO_0000381288Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi195099.CJE1853.

Structurei

3D structure databases

ProteinModelPortaliQ5HSB2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCATEDEA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5HSB2-1 [UniParc]FASTAAdd to Basket

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MQIMLCAISN IASGNCSEDC KYCTQSAHVK TDIQKYRRKE LSQIVLEAKM    50
AKKNEALGFC LVTAGLGLDD EKLEYVCEAA KAVQKEAPNL LLIACNGMAS 100
VEQLKELKKA GIFSYNHNLE SSKEFFPQIC TTHTWESRFQ TNLNAKEAGL 150
MLCCGGIYGM GESEEDRLSF RKSLQELQPF STPINFFIAN ENLKLQVPRL 200
SVDEALKIVR DTKEALPQSV VMVAGGREVV LRERQYEIFQ AGAGAIVIGD 250
YLTTKGEEPS QDIIKLKEMG FTFASECH 278
Length:278
Mass (Da):30,942
Last modified:February 15, 2005 - v1
Checksum:i070B6A0D4F0599DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000025 Genomic DNA. Translation: AAW36275.1.
RefSeqiYP_179823.1. NC_003912.7.

Genome annotation databases

EnsemblBacteriaiAAW36275; AAW36275; CJE1853.
GeneIDi3232480.
KEGGicjr:CJE1853.
PATRICi20045538. VBICamJej134361_1871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000025 Genomic DNA. Translation: AAW36275.1 .
RefSeqi YP_179823.1. NC_003912.7.

3D structure databases

ProteinModelPortali Q5HSB2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 195099.CJE1853.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW36275 ; AAW36275 ; CJE1853 .
GeneIDi 3232480.
KEGGi cjr:CJE1853.
PATRICi 20045538. VBICamJej134361_1871.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CATEDEA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CJEJ195099:GJC0-1892-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM1221.

Entry informationi

Entry nameiBIOB_CAMJR
AccessioniPrimary (citable) accession number: Q5HSB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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