Reviewed, UniProtKB/Swiss-Prot Q5HS07 (THLA_STAEQ)

Names and origin

Protein names

Recommended name:
    Probable acetyl-CoA acyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Ordered Locus Names:

SERP0032

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Probable acetyl-CoA acyltransferase		PRO_0000270509
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	349	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HS07-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: C95DCBE16F213195	FASTA	394	41,595
	10 20 30 40 50 60 MSRIVLAEAY RTPIGVFGGV FKDIPAYELG ATVIRQILEH SQIDPNEINE VILGNVLQAG 70 80 90 100 110 120 QGQNPARIAA IHGGVPEAVP SFTVNKVCGS GLKAIQLAYQ SIVAGDNEIV IAGGMESMSQ 130 140 150 160 170 180 SPMLLKNSRF GFKMGNQTLE DSMIADGLTD KFNDYHMGIT AENLVEQYQI SRKEQDQFAF 190 200 210 220 230 240 DSQQKASRAQ QAGVFDAEIV PVEVPQRKGD PLIISQDEGI RPQTTIDKLA QLRPAFKKDG 250 260 270 280 290 300 SVTAGNASGI NDGAAAMLVM TEDKAKALGL QPIAVLDSFG ASGVAPSIMG IGPVEAIHKA 310 320 330 340 350 360 LKRSNKVIND VDIFELNEAF AAQSIAVNRE LQLPQDKVNV NGGAIALGHP IGASGARTLV 370 380 390 SLLHQLSDAK PTGVASLCIG GGQGIATVVS KYEV

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53454.1.
RefSeq	YP_187632.1.
3D structure databases
HSSP	HSSP built from PDB template 1M3K based on UniProtKB P07097.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HS07.
Genome annotation databases
GeneID	3242978.
GenomeReviews	Gene locus SERP0032 in contig CP000029_GR.
KEGG	ser:SERP0032.
TIGR	SERP0032.
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	DPATMGM.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0032-MONOMER.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THLA_STAEQ

Accession

Primary (citable) accession number: Q5HS07

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)