Q5HRY1 (AHPC_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkyl hydroperoxide reductase subunit C
EC=1.11.1.15

Alternative name(s):
Peroxiredoxin
Thioredoxin peroxidase

Gene names

Name:	ahpC
Ordered Locus Names:	SERP0060

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Directly reduces organic hydroperoxides in its reduced dithiol form By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity.
Post-translational modification	The Cys-49-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-49 (probably Cys-SOH) rapidly reacts with Cys-168-SH of the other subunit to form an intermolecular disulfide. This disulfide is subsequently reduced by thioredoxin By similarity.
Sequence similarities	Belongs to the AhpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Alkyl hydroperoxide reductase subunit C		PRO_0000135130
Regions
	Domain	2 – 159	158	Thioredoxin
Sites
	Active site	49	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	49		Interchain (with C-168); in linked form By similarity
	Disulfide bond	168		Interchain (with C-49); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HRY1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 33183DA3D848D17F	FASTA	189	21,100
	10 20 30 40 50 60 MSLINKEILP FTAQAYDPKK DEFKEVTQED FKGSWNVVCF YPADFSFVCP TELEDLQNQY 70 80 90 100 110 120 AKLQELGVNV YSVSTDTHFV HKAWHDHSDA ISKLEYSMIG DPSQTITRNF DVLDEETGLA 130 140 150 160 170 180 QRGTFIIDPD GVVQAAEINA DGIGRDASTL VNKIKAAQYV RQHPGEVCPA KWEEGSESLQ PGLDLVGKI

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53451.1.
RefSeq	YP_187658.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HRY1.
SMR	Q5HRY1. Positions 2-168.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HRY1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000042920; EBSTAP00000041463; EBSTAG00000042918.
GeneID	3242975.
GenomeReviews	Gene locus SERP0060 in contig CP000029_GR.
KEGG	ser:SERP0060.
NMPDR	fig|176279.3.peg.289.
PATRIC	19610975. VBIStaEpi130894_0054.
TIGR	SERP0060.
Phylogenomic databases
eggNOG	COG0450.
GeneTree	EBGT00050000024736.
HOGENOM	HBG493509.
OMA	GDLADHY.
ProtClustDB	CLSK884603.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0060-MONOMER.
Family and domain databases
InterPro	IPR017559. AhpC. IPR000866. AhpC/TSA. IPR024706. Peroxiredoxin_AhpC-typ. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K03386.
PANTHER	PTHR10681:SF7. PTHR10681:SF7. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
PIRSF	PIRSF000239. AHPC. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR03137. AhpC. 1 hit.
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AHPC_STAEQ

Accession

Primary (citable) accession number: Q5HRY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program