Q5HRU2 (SLE1_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase sle1
EC=3.5.1.28

Gene names

Name:	sle1
Synonyms:	aaa
Ordered Locus Names:	SERP0100

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptidoglycan hydrolase involved in the splitting of the septum during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted By similarity. Cell surface By similarity.
Sequence similarities	Contains 3 LysM repeats. Contains 1 peptidase C51 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell wall biogenesis/degradation Septation Virulence
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 324	299	N-acetylmuramoyl-L-alanine amidase sle1		PRO_0000231627
Regions
	Repeat	29 – 71	43	LysM 1
	Repeat	86 – 128	43	LysM 2
	Repeat	149 – 191	43	LysM 3
	Domain	200 – 324	125	Peptidase C51

Sequences

	Sequence		Length	Mass (Da)
	Q5HRU2 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: AE24C366090455F8	FASTA	324	35,075
	10 20 30 40 50 60 MQKKYITAII GTTALSALAS THAQAATTHT VKSGESVWSI SHKYGISIAK LKSLNGLTSN 70 80 90 100 110 120 LIFPNQVLKV SGSSSRATST NSGTVYTVKA GDSLSSIAAK YGTTYQKIMQ LNGLNNYLIF 130 140 150 160 170 180 PGQKLKVSGK ATSSSRAKAS GSSGRTATYT VKYGDSLSAI ASKYGTTYQK IMQLNGLTNF 190 200 210 220 230 240 FIYPGQKLKV PGGSSSSSSS NNTRSNGGYY SPTFNHQNLY TWGQCTWHVF NRRAEIGKGI 250 260 270 280 290 300 STYWWNANNW DNASAADGYT IDYRPTVGSI AQTDAGYYGH VAFVERVNSD GSILVSEMNW 310 320 SAAPGNMTYR TIPAYQVRNY KFIH

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53528.1.
RefSeq	YP_187697.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HRU2.
ModBase	Search...
Protein family/group databases
CAZy	CBM50. Carbohydrate-Binding Module Family 50.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW53528; AAW53528; SERP0100.
GeneID	3241005.
KEGG	ser:SERP0100.
PATRIC	19611053. VBIStaEpi130894_0093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3942.
HOGENOM	HOG000279823.
OMA	LIASKYN.
ProtClustDB	CLSK884669.
Enzyme and pathway databases
BioCyc	SEPI176279:GJJB-99-MONOMER.
Family and domain databases
InterPro	IPR007921. CHAP. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_subgr. [Graphical view]
Pfam	PF05257. CHAP. 1 hit. PF01476. LysM. 3 hits. [Graphical view]
SMART	SM00257. LysM. 3 hits. [Graphical view]
PROSITE	PS50911. CHAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SLE1_STAEQ

Accession

Primary (citable) accession number: Q5HRU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program