Q5HRP1 (CYSK_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	cysK
Ordered Locus Names:	SERP0152

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Cysteine synthase		PRO_0000167102
Regions
	Region	180 – 184	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	76	1	Pyridoxal phosphate By similarity
	Binding site	268	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HRP1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: E231BA75F40A63EE	FASTA	310	33,152
	10 20 30 40 50 60 MAQKPVDYVT QIIGNTPVVK LRNVVDDDAA DIYVKLEYQN PGGSVKDRIA LAMIEKAERE 70 80 90 100 110 120 GKIKPGDTIV EPTSGNTGIG LAFVCAAKGY KAVFTMPETM SQERRNLLKA YGAELVLTPG 130 140 150 160 170 180 SEAMKGAIKK AKELKEEHGY FEPQQFENPA NPEIHELTTG PELVEQFEGR QIDAFLAGVG 190 200 210 220 230 240 TGGTLSGVGK VLKKEYPNVE IVAIEPEASP VLSGGEPGPH KLQGLGAGFV PDTLNTEVYD 250 260 270 280 290 300 SIIKVGNDTA MDMARRVARE EGILAGISSG AAIYAAIQKA KELGKGKTVV TVLPSNGERY 310 LSTPLYSFDN

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53521.1.
RefSeq	YP_187748.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HRP1.
SMR	Q5HRP1. Positions 9-307.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HRP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000042316; EBSTAP00000040859; EBSTAG00000042314.
GeneID	3240534.
GenomeReviews	Gene locus SERP0152 in contig CP000029_GR.
KEGG	ser:SERP0152.
PATRIC	19611161. VBIStaEpi130894_0143.
TIGR	SERP0152.
Phylogenomic databases
eggNOG	COG0031.
GeneTree	EBGT00050000024422.
HOGENOM	HBG748215.
OMA	SAGANIH.
PhylomeDB	Q5HRP1.
ProtClustDB	CLSK884704.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0152-MONOMER.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_STAEQ

Accession

Primary (citable) accession number: Q5HRP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program