Q5HQX0 (UVRB_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UvrABC system protein B
Short name=Protein UvrB

Alternative name(s):
Excinuclease ABC subunit B

Gene names

Name:	uvrB
Ordered Locus Names:	SERP0426

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204
Subunit structure	Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204
Sequence similarities	Belongs to the UvrB family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Excision nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	SOS response Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC nucleotide-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP excinuclease ABC activity Inferred from electronic annotation. Source: HAMAP helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	UvrABC system protein B HAMAP-Rule MF_00204		PRO_0000138431
Regions
	Domain	28 – 414	387	Helicase ATP-binding
	Domain	432 – 598	167	Helicase C-terminal
	Domain	625 – 660	36	UVR
	Nucleotide binding	41 – 48	8	ATP Potential
	Motif	94 – 117	24	Beta-hairpin HAMAP-Rule MF_00204

Sequences

	Sequence		Length	Mass (Da)
	Q5HQX0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 342D968A43FA1112	FASTA	661	76,669
	10 20 30 40 50 60 MVEHVPFKLK SEFEPQGDQP QAIQKIVDGV NEGKRHQTLL GATGTGKTFT MSNVIKEVGK 70 80 90 100 110 120 PTLIIAHNKT LAGQLYSEFK EFFPENRVEY FVSYYDYYQP EAYVPSTDTF IEKDASINDE 130 140 150 160 170 180 IDQLRHSATS SLFERDDVII IASVSCIYGL GNPEEYKNLV VSVRVGMEME RSELLRKLVD 190 200 210 220 230 240 VQYSRNDIDF QRGTFRVRGD VVEIFPASRE EMCIRVEFFG DEIDRIREVN YLTGEVIRER 250 260 270 280 290 300 EHFTIFPASH FVTREEKMKV AIERIEKELE ERLKELRDEN KLLEAQRLEQ RTNYDLEMMR 310 320 330 340 350 360 EMGFCSGIEN YSVHLTLRPL GSTPYTLLDY FGDDWLVMID ESHVTLPQIR GMYNGDRARK 370 380 390 400 410 420 QVLIDHGFRL PSALDNRPLK FEEFEEKTKQ LVYVSATPGP YELEHTDEMV EQIIRPTGLL 430 440 450 460 470 480 DPKIDVRPTE NQIDDLLSEI QDRVDKDERV LVTTLTKKMS EDLTTYMKEA GIKVNYLHSE 490 500 510 520 530 540 IKTLERIEII RDLRMGTYDA IVGINLLREG IDIPEVSLVV ILDADKEGFL RSDRSLIQTI 550 560 570 580 590 600 GRAARNDKGE VIMYADKITD SMQYAIDETQ RRREIQIAHN KEHGITPKTI NKKIHDVISA 610 620 630 640 650 660 TVESDETNQQ QQTELPKKMT KKERQKTIEN IEKEMKKAAK DLDFEKATEL RDMLFELKAE G

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53840.1.
RefSeq	YP_188019.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HQX0.
SMR	Q5HQX0. Positions 7-654.
ModBase	Search...
Protein-protein interaction databases
STRING	176279.SERP0426.
Proteomic databases
PRIDE	Q5HQX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW53840; AAW53840; SERP0426.
GeneID	3240773.
KEGG	ser:SERP0426.
PATRIC	19611717. VBIStaEpi130894_0405.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0556.
HOGENOM	HOG000073580.
KO	K03702.
OMA	CIYGLGI.
ProtClustDB	PRK05298.
Enzyme and pathway databases
BioCyc	SEPI176279:GJJB-447-MONOMER.
Family and domain databases
Gene3D	4.10.860.10. 1 hit.
HAMAP	MF_00204. UvrB.
InterPro	IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR001943. UVR_dom. IPR004807. UvrB. IPR024759. UvrB_YAD/RRR_dom. [Graphical view]
PANTHER	PTHR24029. PTHR24029. 1 hit.
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. PF02151. UVR. 1 hit. PF12344. UvrB. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF46600. UvrB_C. 1 hit.
TIGRFAMs	TIGR00631. uvrb. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UVRB_STAEQ

Accession

Primary (citable) accession number: Q5HQX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program