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Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11NucleophileUniRule annotation1
Metal bindingi294MagnesiumUniRule annotation1
Metal bindingi356MagnesiumUniRule annotation1
Metal bindingi357MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:SERP0655
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000453041 – 10By similarity10
ChainiPRO_000004530511 – 494AmidophosphoribosyltransferaseAdd BLAST484

Interactioni

Protein-protein interaction databases

STRINGi176279.SERP0655

Structurei

3D structure databases

ProteinModelPortaliQ5HQA0
SMRiQ5HQA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 231Glutamine amidotransferase type-2UniRule annotationAdd BLAST221

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA Bacteria
COG0034 LUCA
HOGENOMiHOG000033688
KOiK00764
OMAiFRPLCLG

Family and domain databases

CDDicd00715 GPATase_N, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.60.20.10, 3 hits
HAMAPiMF_01931 PurF, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR029055 Ntn_hydrolases_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005854 PurF
IPR035584 PurF_N
PfamiView protein in Pfam
PF13537 GATase_7, 1 hit
PF00156 Pribosyltran, 1 hit
PIRSFiPIRSF000485 Amd_phspho_trans, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
SSF56235 SSF56235, 1 hit
TIGRFAMsiTIGR01134 purF, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5HQA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSNHET
60 70 80 90 100
LKGERGLGLL TEAIKDEHMS NIKGYPHAIG HVRYATSGNK GIENIQPFLY
110 120 130 140 150
HFYDMSVGIC HNGNLINAKS LRQNLEEQGA IFHSSSDTEV IMHLIRRSKA
160 170 180 190 200
PTFEEALKES LRLIKGGFTF AILTKDALYG VVDPNAIRPL VVGKMENGAY
210 220 230 240 250
ILASETCAID VLGAEFIQDI HAGEYVVITD EGIEVKTYTR QTTTAISAME
260 270 280 290 300
YIYFARPDST IAGKNVHAVR KASGKRLAQE NPAKADMVIG VPNSSLSAAS
310 320 330 340 350
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK
360 370 380 390 400
DIVLVDDSIV RGTTIKRIVK MLKDSGANRI HVRIASPEFM FPSFYGIDVS
410 420 430 440 450
TTAELISASK SPEEIKNHIG ADSLAYLSVD GLIESIGLDY DAPYHGLCVE
460 470 480 490
SFTGDYPAGL YDYEKNYKKH LSERQKSYIA NNKHYFDSEG NLHV
Length:494
Mass (Da):54,431
Last modified:February 15, 2005 - v1
Checksum:i60CAA1C760B66C12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA Translation: AAW54001.1
RefSeqiWP_002485328.1, NC_002976.3

Genome annotation databases

EnsemblBacteriaiAAW54001; AAW54001; SERP0655
KEGGiser:SERP0655

Similar proteinsi

Entry informationi

Entry nameiPUR1_STAEQ
AccessioniPrimary (citable) accession number: Q5HQA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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