Q5HQA0 (PUR1_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase

Gene names

Name:	purF
Ordered Locus Names:	SERP0655

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.
Cofactor	Binds 1 magnesium ion per subunit.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 10	10	By similarity		PRO_0000045304
	Chain	11 – 494	484	Amidophosphoribosyltransferase		PRO_0000045305
Regions
	Domain	11 – 231	221	Glutamine amidotransferase type-2
Sites
	Active site	11	1	For GATase activity By similarity
	Metal binding	356	1	Magnesium By similarity
	Metal binding	357	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HQA0 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 60CAA1C760B66C12	FASTA	494	54,431
	10 20 30 40 50 60 MSNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSNHET LKGERGLGLL 70 80 90 100 110 120 TEAIKDEHMS NIKGYPHAIG HVRYATSGNK GIENIQPFLY HFYDMSVGIC HNGNLINAKS 130 140 150 160 170 180 LRQNLEEQGA IFHSSSDTEV IMHLIRRSKA PTFEEALKES LRLIKGGFTF AILTKDALYG 190 200 210 220 230 240 VVDPNAIRPL VVGKMENGAY ILASETCAID VLGAEFIQDI HAGEYVVITD EGIEVKTYTR 250 260 270 280 290 300 QTTTAISAME YIYFARPDST IAGKNVHAVR KASGKRLAQE NPAKADMVIG VPNSSLSAAS 310 320 330 340 350 360 GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK DIVLVDDSIV 370 380 390 400 410 420 RGTTIKRIVK MLKDSGANRI HVRIASPEFM FPSFYGIDVS TTAELISASK SPEEIKNHIG 430 440 450 460 470 480 ADSLAYLSVD GLIESIGLDY DAPYHGLCVE SFTGDYPAGL YDYEKNYKKH LSERQKSYIA 490 NNKHYFDSEG NLHV

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54001.1.
RefSeq	YP_188240.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HQA0.
SMR	Q5HQA0. Positions 11-471.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HQA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000042075; EBSTAP00000040618; EBSTAG00000042073.
GeneID	3240495.
GenomeReviews	Gene locus SERP0655 in contig CP000029_GR.
KEGG	ser:SERP0655.
PATRIC	19612183. VBIStaEpi130894_0635.
TIGR	SERP0655.
Phylogenomic databases
eggNOG	COG0034.
GeneTree	EBGT00050000023831.
HOGENOM	HBG392416.
OMA	VWAPGEE.
PhylomeDB	Q5HQA0.
ProtClustDB	PRK07272.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0655-MONOMER.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_STAEQ

Accession

Primary (citable) accession number: Q5HQA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program