Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Phosphoribosylamine--glycine ligase (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11NucleophileUniRule annotation1
Metal bindingi294MagnesiumUniRule annotation1
Metal bindingi356MagnesiumUniRule annotation1
Metal bindingi357MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.A01.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Short name:
GPATaseUniRule annotation
Gene namesi
Name:purFUniRule annotation
Ordered Locus Names:SERP0655
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000453041 – 10By similarity10
ChainiPRO_000004530511 – 494AmidophosphoribosyltransferaseAdd BLAST484

Proteomic databases

PRIDEiQ5HQA0.

Interactioni

Protein-protein interaction databases

STRINGi176279.SERP0655.

Structurei

3D structure databases

ProteinModelPortaliQ5HQA0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 231Glutamine amidotransferase type-2UniRule annotationAdd BLAST221

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Contains 1 glutamine amidotransferase type-2 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5HQA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSNHET
60 70 80 90 100
LKGERGLGLL TEAIKDEHMS NIKGYPHAIG HVRYATSGNK GIENIQPFLY
110 120 130 140 150
HFYDMSVGIC HNGNLINAKS LRQNLEEQGA IFHSSSDTEV IMHLIRRSKA
160 170 180 190 200
PTFEEALKES LRLIKGGFTF AILTKDALYG VVDPNAIRPL VVGKMENGAY
210 220 230 240 250
ILASETCAID VLGAEFIQDI HAGEYVVITD EGIEVKTYTR QTTTAISAME
260 270 280 290 300
YIYFARPDST IAGKNVHAVR KASGKRLAQE NPAKADMVIG VPNSSLSAAS
310 320 330 340 350
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK
360 370 380 390 400
DIVLVDDSIV RGTTIKRIVK MLKDSGANRI HVRIASPEFM FPSFYGIDVS
410 420 430 440 450
TTAELISASK SPEEIKNHIG ADSLAYLSVD GLIESIGLDY DAPYHGLCVE
460 470 480 490
SFTGDYPAGL YDYEKNYKKH LSERQKSYIA NNKHYFDSEG NLHV
Length:494
Mass (Da):54,431
Last modified:February 15, 2005 - v1
Checksum:i60CAA1C760B66C12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW54001.1.
RefSeqiWP_002485328.1. NC_002976.3.

Genome annotation databases

EnsemblBacteriaiAAW54001; AAW54001; SERP0655.
KEGGiser:SERP0655.
PATRICi19612183. VBIStaEpi130894_0635.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW54001.1.
RefSeqiWP_002485328.1. NC_002976.3.

3D structure databases

ProteinModelPortaliQ5HQA0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176279.SERP0655.

Protein family/group databases

MEROPSiC44.A01.

Proteomic databases

PRIDEiQ5HQA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW54001; AAW54001; SERP0655.
KEGGiser:SERP0655.
PATRICi19612183. VBIStaEpi130894_0635.

Phylogenomic databases

eggNOGiENOG4105CBA. Bacteria.
COG0034. LUCA.
HOGENOMiHOG000033688.
KOiK00764.
OMAiIRHFGVK.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUR1_STAEQ
AccessioniPrimary (citable) accession number: Q5HQA0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.