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Q5HQA0 (PUR1_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
Ordered Locus Names:SERP0655
OrganismStaphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]
Taxonomic identifier176279 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1010 By similarity
PRO_0000045304
Chain11 – 494484Amidophosphoribosyltransferase
PRO_0000045305

Regions

Domain11 – 231221Glutamine amidotransferase type-2

Sites

Active site111For GATase activity By similarity
Metal binding3561Magnesium By similarity
Metal binding3571Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HQA0 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 60CAA1C760B66C12

FASTA49454,431
        10         20         30         40         50         60 
MSNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSNHET LKGERGLGLL 

        70         80         90        100        110        120 
TEAIKDEHMS NIKGYPHAIG HVRYATSGNK GIENIQPFLY HFYDMSVGIC HNGNLINAKS 

       130        140        150        160        170        180 
LRQNLEEQGA IFHSSSDTEV IMHLIRRSKA PTFEEALKES LRLIKGGFTF AILTKDALYG 

       190        200        210        220        230        240 
VVDPNAIRPL VVGKMENGAY ILASETCAID VLGAEFIQDI HAGEYVVITD EGIEVKTYTR 

       250        260        270        280        290        300 
QTTTAISAME YIYFARPDST IAGKNVHAVR KASGKRLAQE NPAKADMVIG VPNSSLSAAS 

       310        320        330        340        350        360 
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK DIVLVDDSIV 

       370        380        390        400        410        420 
RGTTIKRIVK MLKDSGANRI HVRIASPEFM FPSFYGIDVS TTAELISASK SPEEIKNHIG 

       430        440        450        460        470        480 
ADSLAYLSVD GLIESIGLDY DAPYHGLCVE SFTGDYPAGL YDYEKNYKKH LSERQKSYIA 

       490 
NNKHYFDSEG NLHV 

« Hide

References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000029 Genomic DNA. Translation: AAW54001.1.
RefSeqYP_188240.1. NC_002976.3.

3D structure databases

ProteinModelPortalQ5HQA0.
SMRQ5HQA0. Positions 11-471.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176279.SERP0655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW54001; AAW54001; SERP0655.
GeneID3240495.
KEGGser:SERP0655.
PATRIC19612183. VBIStaEpi130894_0635.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0034.
HOGENOMHOG000033688.
KOK00764.
OMASGIPYEM.
OrthoDBEOG6KT2Q1.

Enzyme and pathway databases

BioCycSEPI176279:GJJB-680-MONOMER.
UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_STAEQ
AccessionPrimary (citable) accession number: Q5HQA0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways