Q5HQ96 (PUR2_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	SERP0659

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151485
Regions
	Domain	108 – 310	203	ATP-grasp
	Nucleotide binding	134 – 190	57	ATP By similarity
Sites
	Metal binding	280	1	Magnesium or manganese By similarity
	Metal binding	282	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HQ96 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 4EEA202347FD889A	FASTA	413	45,326
	10 20 30 40 50 60 MKVLIVGAGG REHALASKIN QSPIVDKVYA IPGNDAMVNI AEVHSEIAES DHQAILHFAQ 70 80 90 100 110 120 HNAIDWVIIG PEQPLIDGLA DLLRNANIKV FGPGQDAAQI EGSKLFAKQL MDKYRIPTAE 130 140 150 160 170 180 YKEVSSRNEA LQYVETCDLP IVIKKDGLAA GKGVIIAFTR EDALDGVKKI YQEEKGKVVF 190 200 210 220 230 240 ESYLEGEEFS LMTFVNGDYA VPFDCIAQDH KRAFDNDQGP NTGGMGAYCP VPHIDASVLE 250 260 270 280 290 300 QTNKEIAQPI AKAMAQEGHD FFGLLYIGAI LTKDGPKVIE FNARFGDPEA QVLLTRLESD 310 320 330 340 350 360 LMQLIIDLEN CQPIHFNWKD EAVVGVMLAS KGYPGSYEKG HEISGFNLDS HYYVSGLKKE 370 380 390 400 410 GQCFVNSGGR VILAIGEGPT VEKAQANAYE HARQIKSDNL FYRNDIGNKA ITK

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54012.1.
RefSeq	YP_188244.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HQ96.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HQ96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000042094; EBSTAP00000040637; EBSTAG00000042092.
GeneID	3240883.
GenomeReviews	Gene locus SERP0659 in contig CP000029_GR.
KEGG	ser:SERP0659.
PATRIC	19612191. VBIStaEpi130894_0639.
TIGR	SERP0659.
Phylogenomic databases
eggNOG	COG0151.
GeneTree	EBGT00050000023921.
HOGENOM	HBG404060.
OMA	MGAYTPL.
PhylomeDB	Q5HQ96.
ProtClustDB	PRK13790.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0659-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01945.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. PurD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_STAEQ

Accession

Primary (citable) accession number: Q5HQ96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program