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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).By similarity

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei554Aminoacyl-adenylateBy similarity1
Binding sitei598ATPBy similarity1
Metal bindingi885ZincBy similarity1
Metal bindingi888ZincBy similarity1
Metal bindingi905ZincBy similarity1
Metal bindingi908ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSEPI176279:G1G46-785-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SERP0758
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000531 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984721 – 916Isoleucine--tRNA ligaseAdd BLAST916

Proteomic databases

PRIDEiQ5HPZ9

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi176279.SERP0758

Structurei

3D structure databases

ProteinModelPortaliQ5HPZ9
SMRiQ5HPZ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi595 – 599"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) (By similarity).By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q5HPZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYKDTLLMP KTDFPMRGGL PNKEPQIQEM WDNDDQYRKA LEKNKNNPSF
60 70 80 90 100
ILHDGPPYAN GNLHMGHALN KIIKDFIVRY KTMQGFYAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKKGV DRKKMSVAEF REKCKEFALK QIDIQKKDFK RLGVRGDFNN
160 170 180 190 200
PYITLTPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
HDKRSASIYV AFDVKDSKGK VDSDAQFIIW TTTPWTIPSN VAITVHPELK
260 270 280 290 300
YGQYNVDGHK YIVAQALSEE VAEALGWDKD SIQLEKEFTG KELEFVEAQH
310 320 330 340 350
PFLDRVSLVI NGEHVTTDAG TGCVHTAPGH GEDDYIVGQK YDLPVISPLN
360 370 380 390 400
DKGVFTEEGG PFEGMFYDKA NKAVTDLLKE KDALLKLDFI THSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASINKVR QDILDAIEDT NFKVDWGKTR IYNMIRDRGE
460 470 480 490 500
WVISRQRVWG VPLPVFYAEN GDIIMTKETV NHVADLFEKH GSNIWFEKEA
510 520 530 540 550
KELLPEGFSH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE TRPELSFPAD
560 570 580 590 600
LYFEGSDQYR GWFNSSITTA VATRGQAPYK FLLSHGFVMD GEGKKMSKSL
610 620 630 640 650
GNVIVPDQVV KQKGADIARL WVSSTDYLSD VRISDEILKQ TSDVYRKIRN
660 670 680 690 700
TLRFMLGNIN DFNPETDSIA ETNLLEVDRY LLNRLREFTA STINNYENFD
710 720 730 740 750
YLNIYQEVQN FINVELSNFY LDYGKDILYI EKKDSHKRRS MQTVLYQILV
760 770 780 790 800
DMTKLLAPIL VHTAEEVWSH TPHVKEESVH LSDMPKVVDV DEELLEKWNT
810 820 830 840 850
FMNLRDDVNR ALEQARNEKV IGKSLEAKVV IGSNESFNTA EFLQQFNDLQ
860 870 880 890 900
QLFIVSQVEV KDKVNDGVSY QYGDIHIKHA EGEKCERCWN YTEELGSVGE
910
LEHLCPRCQE VVKTLV
Length:916
Mass (Da):105,164
Last modified:February 15, 2005 - v1
Checksum:iE2703272E7420212
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA Translation: AAW54153.1
RefSeqiWP_002490488.1, NC_002976.3

Genome annotation databases

EnsemblBacteriaiAAW54153; AAW54153; SERP0758
KEGGiser:SERP0758

Similar proteinsi

Entry informationi

Entry nameiSYI_STAEQ
AccessioniPrimary (citable) accession number: Q5HPZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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