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Q5HPZ9

- SYI_STAEQ

UniProt

Q5HPZ9 - SYI_STAEQ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.By similarity

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateBy similarity
    Binding sitei598 – 5981ATPBy similarity
    Metal bindingi885 – 8851ZincBy similarity
    Metal bindingi888 – 8881ZincBy similarity
    Metal bindingi905 – 9051ZincBy similarity
    Metal bindingi908 – 9081ZincBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSEPI176279:GJJB-784-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:ileS
    Ordered Locus Names:SERP0758
    OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
    Taxonomic identifieri176279 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000531: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 916916Isoleucine--tRNA ligasePRO_0000098472Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi176279.SERP0758.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5HPZ9.
    SMRiQ5HPZ9. Positions 1-916.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5HPZ9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYKDTLLMP KTDFPMRGGL PNKEPQIQEM WDNDDQYRKA LEKNKNNPSF    50
    ILHDGPPYAN GNLHMGHALN KIIKDFIVRY KTMQGFYAPY VPGWDTHGLP 100
    IEQALTKKGV DRKKMSVAEF REKCKEFALK QIDIQKKDFK RLGVRGDFNN 150
    PYITLTPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
    HDKRSASIYV AFDVKDSKGK VDSDAQFIIW TTTPWTIPSN VAITVHPELK 250
    YGQYNVDGHK YIVAQALSEE VAEALGWDKD SIQLEKEFTG KELEFVEAQH 300
    PFLDRVSLVI NGEHVTTDAG TGCVHTAPGH GEDDYIVGQK YDLPVISPLN 350
    DKGVFTEEGG PFEGMFYDKA NKAVTDLLKE KDALLKLDFI THSYPHDWRT 400
    KKPVIFRATP QWFASINKVR QDILDAIEDT NFKVDWGKTR IYNMIRDRGE 450
    WVISRQRVWG VPLPVFYAEN GDIIMTKETV NHVADLFEKH GSNIWFEKEA 500
    KELLPEGFSH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE TRPELSFPAD 550
    LYFEGSDQYR GWFNSSITTA VATRGQAPYK FLLSHGFVMD GEGKKMSKSL 600
    GNVIVPDQVV KQKGADIARL WVSSTDYLSD VRISDEILKQ TSDVYRKIRN 650
    TLRFMLGNIN DFNPETDSIA ETNLLEVDRY LLNRLREFTA STINNYENFD 700
    YLNIYQEVQN FINVELSNFY LDYGKDILYI EKKDSHKRRS MQTVLYQILV 750
    DMTKLLAPIL VHTAEEVWSH TPHVKEESVH LSDMPKVVDV DEELLEKWNT 800
    FMNLRDDVNR ALEQARNEKV IGKSLEAKVV IGSNESFNTA EFLQQFNDLQ 850
    QLFIVSQVEV KDKVNDGVSY QYGDIHIKHA EGEKCERCWN YTEELGSVGE 900
    LEHLCPRCQE VVKTLV 916
    Length:916
    Mass (Da):105,164
    Last modified:February 15, 2005 - v1
    Checksum:iE2703272E7420212
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW54153.1.
    RefSeqiYP_188341.1. NC_002976.3.

    Genome annotation databases

    EnsemblBacteriaiAAW54153; AAW54153; SERP0758.
    GeneIDi3242826.
    KEGGiser:SERP0758.
    PATRICi19612389. VBIStaEpi130894_0737.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW54153.1 .
    RefSeqi YP_188341.1. NC_002976.3.

    3D structure databases

    ProteinModelPortali Q5HPZ9.
    SMRi Q5HPZ9. Positions 1-916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176279.SERP0758.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW54153 ; AAW54153 ; SERP0758 .
    GeneIDi 3242826.
    KEGGi ser:SERP0758.
    PATRICi 19612389. VBIStaEpi130894_0737.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SEPI176279:GJJB-784-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
      Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
      , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
      J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35984 / RP62A.

    Entry informationi

    Entry nameiSYI_STAEQ
    AccessioniPrimary (citable) accession number: Q5HPZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3