Q5HPC4 (ARLS_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal transduction histidine-protein kinase ArlS
EC=2.7.13.3

Gene names

Name:	arlS
Ordered Locus Names:	SERP0988

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Member of the two-component regulatory system ArlS/ArlR. ArlS probably functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to ArlR By similarity.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Signal transduction histidine-protein kinase ArlS		PRO_0000293450
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	157 – 177	21	Helical; Potential
	Domain	179 – 232	54	HAMP
	Domain	240 – 456	217	Histidine kinase
Amino acid modifications
	Modified residue	243	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HPC4 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: BF6EEFFE13E92013	FASTA	456	52,883
	10 20 30 40 50 60 MIKRQKLKYK WMLITTLITF TTILLFCLII IFFLKDTLRS SEIDEAERSS NDIANLFHSK 70 80 90 100 110 120 SLSDISALDL NASLENFQEI LIYDDKGRKL IQTSNDNTLA YDNKIDFKHP ERIHIHRSHG 130 140 150 160 170 180 INYLVITEPI RSKEFSGYSV LVHSLQNYDN LVKSLYIVAL AFGLIATIIT AGVSYIFSSQ 190 200 210 220 230 240 ITKPIVTMSN KMNQIRRDGF QNKLELTTNY EETDNLIDTF NEMMYQIEES FNQQRQFVED 250 260 270 280 290 300 ASHELRTPLQ IIQGHLNLIQ RWGKKDPAVL EESLNISIEE VNRITKLVEE LLLLTKDRVN 310 320 330 340 350 360 HNVLECENVD INSEIQSRVK SLQHLHPDYT FETHLATKPI QLKINRHQFE QLLLIFIDNA 370 380 390 400 410 420 MKYDTEHKHI KIVTQLKNKM IMIDITDHGM GIPKADLEFI FDRFYRVDKS RARSQGGNGL 430 440 450 GLSIAEKIVQ LNGGMIQVES ELQNYTTFKI SFPVLN

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54342.1.
RefSeq	YP_188566.1. NC_002976.3.
3D structure databases
HSSP	HSSP built from PDB template 2C2A based on UniProtKB Q9WZV7.
ProteinModelPortal	Q5HPC4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HPC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000043019; EBSTAP00000041562; EBSTAG00000043017.
GeneID	3242088.
GenomeReviews	Gene locus SERP0988 in contig CP000029_GR.
KEGG	ser:SERP0988.
NMPDR	fig|176279.3.peg.1066.
PATRIC	19612843. VBIStaEpi130894_0964.
TIGR	SERP0988.
Phylogenomic databases
eggNOG	COG0642.
GeneTree	EBGT00050000023863.
HOGENOM	HBG694527.
OMA	FSSQITK.
ProtClustDB	CLSK885336.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP0988-MONOMER.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR003660. HAMP_linker_domain. IPR004358. Sig_transdc_His_kin-like_C. IPR003661. Sig_transdc_His_kin_sub1_dim/P. IPR005467. Sig_transdc_His_kinase_core. IPR009082. Sig_transdc_His_kinase_dimeric. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF00672. HAMP. 1 hit. PF02518. HATPase_c. 1 hit. PF00512. HisKA. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00304. HAMP. 1 hit. SM00387. HATPase_c. 1 hit. SM00388. HisKA. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF47384. His_kin_homodim. 1 hit.
PROSITE	PS50885. HAMP. 1 hit. PS50109. HIS_KIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLS_STAEQ

Accession

Primary (citable) accession number: Q5HPC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program