Q5HNV1 (AROE_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	SERP1163

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 269

269

Shikimate dehydrogenase HAMAP MF_00222

PRO_0000136037

Regions

Nucleotide binding

124 – 128

5

NADP By similarity

Sites

Active site

64

1

Proton acceptor Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

269

Helix Strand Turn

Details...

Beta strand

2 – 6

5

Helix

15 – 25

11

Beta strand

31 – 34

4

Helix

39 – 44

6

Helix

45 – 51

7

Turn

64 – 67

4

Helix

68 – 70

3

Helix

76 – 80

5

Turn

109 – 111

3

Beta strand

120 – 123

4

Helix

133 – 137

5

Beta strand

145 – 147

3

Helix

151 – 154

4

Beta strand

162 – 164

3

Helix

166 – 171

6

Helix

173 – 175

3

Beta strand

177 – 181

5

Beta strand

205 – 209

5

Beta strand

212 – 215

4

Helix

217 – 224

8

Helix

234 – 238

5

Helix

242 – 248

7

Helix

254 – 265

12

Sequences

	Sequence		Length	Mass (Da)
	Q5HNV1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 9F9808764B9C21C4	FASTA	269	29,947
	10 20 30 40 50 60 MKFAVIGNPI SHSLSPLMHH ANFQSLNLEN TYEAINVPVN QFQDIKKIIS EKSIDGFNVT 70 80 90 100 110 120 IPHKERIIPY LDDINEQAKS VGAVNTVLVK DGKWIGYNTD GIGYVNGLKQ IYEGIEDAYI 130 140 150 160 170 180 LILGAGGASK GIANELYKIV RPTLTVANRT MSRFNNWSLN INKINLSHAE SHLDEFDIII 190 200 210 220 230 240 NTTPAGMNGN TDSVISLNRL ASHTLVSDIV YNPYKTPILI EAEQRGNPIY NGLDMFVHQG 250 260 AESFKIWTNL EPDIKAMKNI VIQKLKGEL

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

CP000029 Genomic DNA. Translation: AAW54512.1.

RefSeq

YP_188739.1. NC_002976.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DON	X-ray	2.10	A	1-269	[»]
3DOO	X-ray	2.20	A	1-269	[»]

ProteinModelPortal

Q5HNV1.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5HNV1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBSTAT00000042545; EBSTAP00000041088; EBSTAG00000042543.

GeneID

3240969.

GenomeReviews

Gene locus SERP1163 in contig CP000029_GR.

KEGG

ser:SERP1163.

PATRIC

19613179. VBIStaEpi130894_1132.

TIGR

SERP1163.

Phylogenomic databases

eggNOG

COG0169.

GeneTree

EBGT00050000025479.

HOGENOM

HBG553408.

OMA

GIANELY.

PhylomeDB

Q5HNV1.

ProtClustDB

CLSK885454.

Enzyme and pathway databases

BioCyc

SEPI176279:SERP1163-MONOMER.

Family and domain databases

HAMAP

MF_00222. Shikimate_DH_AroE.
[Tree]

InterPro

IPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_quinate_DH.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K00014.

Pfam

PF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00507. AroE. 1 hit.

ProtoNet

Search...

Entry information

Entry name

AROE_STAEQ

Accession

Primary (citable) accession number: Q5HNV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program