Q5HNR8 (RELA_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I

Gene names

Name:	relA
Ordered Locus Names:	SERP1196

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	729 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Pathway	Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.
Sequence similarities	Belongs to the RelA/SpoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	guanosine tetraphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 729	729	GTP pyrophosphokinase		PRO_0000166562
Regions
	Domain	50 – 149	100	HD
	Domain	654 – 727	74	ACT

Sequences

	Sequence		Length	Mass (Da)
	Q5HNR8 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 2EEE5C9BEF92E0DA	FASTA	729	83,468
	10 20 30 40 50 60 MNNEYPYSAD EVLYKAKSYL STSEYEYVLK SYHIAYEAHK GQFRKNGLPY IMHPIQVAGI 70 80 90 100 110 120 LTEMRLDGPT IVAGFLHDVI EDTSYTFEDV KDMFNEEIAR IVDGVTKLKK VKYRSKEEQQ 130 140 150 160 170 180 AENHRKLFIA IAKDVRVILV KLADRLHNMR TLKAMPREKQ VRISKETLEI YAPLAHRLGI 190 200 210 220 230 240 NTIKWELEDT ALRYIDSVQY FRIVNLMKKK RSEREAYITN AINKIKNEMT KMNLSGEING 250 260 270 280 290 300 RPKHIYSIYR KMIKQKKQFD QIFDLLAIRI IVNSINDCYA TLGLVHTLWK PMPGRFKDYI 310 320 330 340 350 360 AMPKQNMYQS LHTTVVGPNG DPLEIQIRTH EMHEIAEHGV AAHWAYKEGK TVNQKTQDFQ 370 380 390 400 410 420 NKLNWLKELA ETDHTSADAQ EFMESLKYDL QSDKVYAFTP ASDVIELPYG AVPIDFAYAI 430 440 450 460 470 480 HSEVGNKMIG AKVNGKIVPI DYVLQTGDII EIRTSKHSYG PSRDWLKIVK SSSAKSKIKS 490 500 510 520 530 540 FFKKQDRSSN IEKGKFMVEA EIKEQGFRVE DILTEKNLEV VNEKYHFAND EDLYAAVGFG 550 560 570 580 590 600 GVTSIQIVNK LTERQRILDK QKALNEAQEV TKSVPIKKDI TTDSGVYVEG LENVLIKLSK 610 620 630 640 650 660 CCNPIPGDDI VGYITKGHGI KVHRTDCPNI KNETDRLISV EWVKSKDSTQ QYQVDLEVTA 670 680 690 700 710 720 YDRNGLLNEV LQAVNSTAGS LIKVSGRSDI DKNAVINISV MVKNVNDVYR VVEKIKQLGD VYTVSRVWN

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54575.1.
RefSeq	YP_188772.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HNR8.
SMR	Q5HNR8. Positions 7-350.
ModBase	Search...
Protein-protein interaction databases
STRING	176279.SERP1196.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW54575; AAW54575; SERP1196.
GeneID	3241831.
KEGG	ser:SERP1196.
PATRIC	19613245. VBIStaEpi130894_1165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0317.
HOGENOM	HOG000018301.
KO	K00951.
OMA	LSWFREI.
ProtClustDB	CLSK885475.
Enzyme and pathway databases
BioCyc	SEPI176279:GJJB-1223-MONOMER.
UniPathway	UPA00908; UER00884.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR026020. (p)ppGpp_Synthase. IPR012675. Beta-grasp_dom. IPR003607. HD/PDEase_dom. IPR004811. RelA/Spo_fam. IPR007685. RelA_SpoT. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. spoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RELA_STAEQ

Accession

Primary (citable) accession number: Q5HNR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program