Reviewed, UniProtKB/Swiss-Prot Q5HNJ6 (DHA_STAEQ)

Names and origin

Protein names

Recommended name:
    Alanine dehydrogenase
    EC=1.4.1.1

Gene names

Name:	ald
Ordered Locus Names:	SERP1272

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer By similarity.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Alanine dehydrogenase		PRO_0000199008
Regions
	Nucleotide binding	169 – 199	31	NAD By similarity
Sites
	Active site	95	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5HNJ6-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 78541923F8E7CD6F	FASTA	371	40,195
	10 20 30 40 50 60 MKIGIPKEIK NNENRVGLSP SGVHALVDQG HEVLVETNAG LGSYFEDGDY QEAGAKIVDE 70 80 90 100 110 120 QSKAWDVDMV IKVKEPLESE YKFFKEELIL FTYLHLANEQ KLTQALVDNK VISIAYETVQ 130 140 150 160 170 180 LPDGSLPLLT PMSEVAGRMS TQVGAEFLQR FNGGMGILLG GIPGVPKGKV TIIGGGQAGT 190 200 210 220 230 240 NAAKIALGLG AEVTILDVNP KRLEELEDLF DGRVRTIMSN PLNIEMYVKE SDLVIGAVLI 250 260 270 280 290 300 PGAKAPNLVT EDMIKEMKDG SVIVDIAIDQ GGIFETTDKI TTHDNPTYTK HGVVHYAVAN 310 320 330 340 350 360 MPGAVPRTST IGLNNATLPY AQLLANKGYR EAFKVNHPLS LGLNTFNGHV TNKNVADTFN 370 FEYTSIEDAL K

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54667.1.
RefSeq	YP_188845.1.
3D structure databases
SMR	Q5HNJ6. Positions 1-359.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HNJ6.
Genome annotation databases
GeneID	3241718.
GenomeReviews	Gene locus SERP1272 in contig CP000029_GR.
KEGG	ser:SERP1272.
TIGR	SERP1272.
Phylogenomic databases
eggNOG	COG0686.
HOGENOM	HBG289735.
OMA	VAHGHEV.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP1272-MONOMER.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR008141. Ala_DH/Pyr_transHydgase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. alaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA_STAEQ

Accession

Primary (citable) accession number: Q5HNJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)