Reviewed, UniProtKB/Swiss-Prot Q5HN85 (FUMC_STAEQ)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	SERP1387

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161318
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HN85-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 478E748E9F13827A	FASTA	461	51,329
	10 20 30 40 50 60 MSVRIEHDTF GEIEVPEDKY WGAQTERSKR NFPVGKEHMP IQVIYGFAQL KRGAALANHE 70 80 90 100 110 120 LGKLSDEKKN AIVYACDRIL NGELDNHFPL VIWQTGSGTQ SNMNVNEVVS YVANEYLKKH 130 140 150 160 170 180 GSKETIHPND DVNKSQSSND TFPTAMHVAL FHEVETKLEP ALNHLRQTFK EKEDQYQSII 190 200 210 220 230 240 KIGRTHLQDA TPIKLGQEIS GWRYMLEKCE QLLSESKKHI LNLAIGGTAV GTGINAHPEF 250 260 270 280 290 300 GHKVAKYISQ NTGYAFVSSE NKFHALTSHD EIVQLHGTLK ALATDLMKIA NDIRWLASGP 310 320 330 340 350 360 RAGLAEISIP ENEPGSSIMP GKVNPTQCEM LTMVAVQVMG NDTTVGIASS QGNFELNVFK 370 380 390 400 410 420 PVIMHNTLQS IYLLADGMNT FNKNCAIGIQ PIEENINNYL NQSLMLVTAL NPHIGYEKAA 430 440 450 460 QIAKKAHKEG LTLKESAIES GYVTESQFEE WIKPEDMVDP H

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000029 Genomic DNA. Translation: AAW54715.1.
RefSeq	YP_188956.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HN85.
Genome annotation databases
GeneID	3241451.
GenomeReviews	Gene locus SERP1387 in contig CP000029_GR.
KEGG	ser:SERP1387.
TIGR	SERP1387.
Phylogenomic databases
HOGENOM	Q5HN85.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP1387-MON.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_STAEQ

Accession

Primary (citable) accession number: Q5HN85

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)