Reviewed, UniProtKB/Swiss-Prot Q5HN75 (GSEA_STAEQ)

Names and origin

Protein names

Recommended name:
    Glutamyl endopeptidase
    EC=3.4.21.19
Alternative name(s):
    Glutamic acid-specific protease
      Short name=GluSE

Gene names

Name:	gseA
Ordered Locus Names:	SERP1397

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits a significant hydrolytic activity for the carbonyl side of glutamic acid. Shows activity toward human fibronectin and type 1 collagen By similarity.
Catalytic activity	Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase S1B family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 66	39	By similarity		PRO_0000026898
	Chain	67 – 282	216	Glutamyl endopeptidase		PRO_0000026899
Sites
	Active site	117	1	Charge relay system By similarity
	Active site	159	1	Charge relay system By similarity
	Active site	235	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HN75-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 5DA679C179F8A787	FASTA	282	30,831
	10 20 30 40 50 60 MKKRFLSICT MTIAALATTT MVNTSYAKTD TESHNHSSLG TENKNVLDIN SSSHNIKPSQ 70 80 90 100 110 120 NKSYPSVILP NNNRHQIFNT TQGHYDAVSF IYIPIHGGYM SGSGVVVGEN EILTNKHVVN 130 140 150 160 170 180 GAKGNPRNIS VHPSAKNEND YPNGKFVGQE IIPYPGNSDL AILRVSPNEH NQHIGQVVKP 190 200 210 220 230 240 ATISSNTDTR INENITVTGY PGDKPLATMW ESVGKVVYIG GEELRYDLST VGGNSGSPVF 250 260 270 280 NGKNQVIGIH YGGVDNKYNS SVYINDFVQQ FLRNNIPDIN IQ

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW54738.1.
RefSeq	YP_188966.1.
3D structure databases
SMR	Q5HN75. Positions 67-281.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HN75.
Genome annotation databases
GeneID	3240839.
GenomeReviews	Gene locus SERP1397 in contig CP000029_GR.
KEGG	ser:SERP1397.
NMPDR	fig|176279.3.peg.1366.
TIGR	SERP1397.
Phylogenomic databases
eggNOG	COG3591.
HOGENOM	HBG693141.
OMA	EDINFAN.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP1397-MONOMER.
Family and domain databases
InterPro	IPR000126. Pept_S1B_AS. IPR001254. Peptidase_S1_S6. IPR008256. Peptidase_S1B. IPR008353. Peptidase_S1B_tx. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR01774. EXFOLTOXIN. PR00839. V8PROTEASE.
PROSITE	PS00672. V8_HIS. False negative. PS00673. V8_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSEA_STAEQ

Accession

Primary (citable) accession number: Q5HN75

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	February 15, 2005
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)