Reviewed, UniProtKB/Swiss-Prot Q5HN46 (AMPM_STAEQ)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	SERP1426

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Methionine aminopeptidase		PRO_0000148962
Sites
	Metal binding	93	1	Cobalt 1 By similarity
	Metal binding	104	1	Cobalt 1 By similarity
	Metal binding	104	1	Cobalt 2 By similarity
	Metal binding	168	1	Cobalt 2 By similarity
	Metal binding	202	1	Cobalt 2 By similarity
	Metal binding	233	1	Cobalt 1 By similarity
	Metal binding	233	1	Cobalt 2 By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HN46-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: A105F719EC8571E5	FASTA	251	27,531
	10 20 30 40 50 60 MIVKTDEELQ ALKEIGYICA KVRDTMKEAT KPGVTTRELD HIAKDLFEEH GAISAPIHDE 70 80 90 100 110 120 NFPGQTCISV NEEVAHGIPG KRVIHEGDLV NIDVSALKNG YYADTGISFV VGKSDQPLKQ 130 140 150 160 170 180 KVCDVATMAF ENAMKKVKPG TKLSNIGKAV HATARQNDLT VIKNLTGHGV GQSLHEAPNH 190 200 210 220 230 240 VMNYFDPKDK TLLKEGQVIA VEPFISTHAT FVTEGKNEWA FETKDKSYVA QIEHTVIVTK 250 DGPLLTTKID D

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000029 Genomic DNA. Translation: AAW54790.1.
RefSeq	YP_188995.1.
3D structure databases
SMR	Q5HN46. Positions 1-249.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HN46.
Genome annotation databases
GeneID	3241924.
GenomeReviews	Gene locus SERP1426 in contig CP000029_GR.
KEGG	ser:SERP1426.
NMPDR	fig|176279.3.peg.1395.
TIGR	SERP1426.
Phylogenomic databases
HOGENOM	Q5HN46.
OMA	PGATCIS.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP1426-MON.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMPM_STAEQ

Accession

Primary (citable) accession number: Q5HN46

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	February 15, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)