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Q5HN36

- DAGK_STAEQ

UniProt

Q5HN36 - DAGK_STAEQ

Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway By similarity.By similarity

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411ATPPROSITE-ProRule annotation
    Binding sitei94 – 941ATPPROSITE-ProRule annotation
    Metal bindingi213 – 2131Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi216 – 2161MagnesiumBy similarity
    Metal bindingi218 – 2181Magnesium; via carbonyl oxygenBy similarity
    Active sitei273 – 2731Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 145ATPPROSITE-ProRule annotation
    Nucleotide bindingi67 – 737ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. diacylglycerol kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. phospholipid biosynthetic process Source: UniProtKB-KW
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSEPI176279:GJJB-1500-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase (EC:2.7.1.107)
    Short name:
    DAG kinase
    Short name:
    DAGK
    Gene namesi
    Name:dagK
    Ordered Locus Names:SERP1436
    OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
    Taxonomic identifieri176279 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000531: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316Diacylglycerol kinasePRO_0000386500Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi176279.SERP1436.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5HN36.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 132132DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the diacylglycerol/lipid kinase family.Curated
    Contains 1 DAGKc domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1597.
    HOGENOMiHOG000261395.
    KOiK07029.
    OMAiYLKGMEM.
    OrthoDBiEOG613098.

    Family and domain databases

    Gene3Di3.40.50.10330. 1 hit.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view]
    PfamiPF00781. DAGK_cat. 1 hit.
    [Graphical view]
    SMARTiSM00046. DAGKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.
    TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
    PROSITEiPS50146. DAGK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5HN36-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKRARIIYN PTSGKELFKR VLPDALIKLE KAGYETSAYA TEKIGDATFE    50
    AERALESEYD LLIAAGGDGT LNEVVNGIAE QPNRPKLGVI PMGTVNDFGR 100
    ALHLPSDIMG AIDVIIDGHT TKVDIGKMNN RYFINLAAGG KLTQVSYETP 150
    SKLKSIVGPF AYYIKGFEML PQMKAVDVRI EYDDNIFQGE ALLFLLGLTN 200
    SMAGFEKLVP DAKLDDGYFT LIIVEKANLA ELGHIMTLAS RGEHTKHPKV 250
    IYAKAKSINI SSFTDMQLNV DGEYGGKLPA NFLNLEQHIE IFTPKDVFNE 300
    ELLENDTITD ITPDKQ 316
    Length:316
    Mass (Da):34,950
    Last modified:February 15, 2005 - v1
    Checksum:iA3F39142BEB35A53
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW54816.1.
    RefSeqiYP_189005.1. NC_002976.3.

    Genome annotation databases

    EnsemblBacteriaiAAW54816; AAW54816; SERP1436.
    GeneIDi3241949.
    KEGGiser:SERP1436.
    PATRICi19613801. VBIStaEpi130894_1404.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW54816.1 .
    RefSeqi YP_189005.1. NC_002976.3.

    3D structure databases

    ProteinModelPortali Q5HN36.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176279.SERP1436.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW54816 ; AAW54816 ; SERP1436 .
    GeneIDi 3241949.
    KEGGi ser:SERP1436.
    PATRICi 19613801. VBIStaEpi130894_1404.

    Phylogenomic databases

    eggNOGi COG1597.
    HOGENOMi HOG000261395.
    KOi K07029.
    OMAi YLKGMEM.
    OrthoDBi EOG613098.

    Enzyme and pathway databases

    BioCyci SEPI176279:GJJB-1500-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.10330. 1 hit.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR005218. Diacylglycerol/lipid_kinase.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00781. DAGK_cat. 1 hit.
    [Graphical view ]
    SMARTi SM00046. DAGKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
    PROSITEi PS50146. DAGK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
      Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
      , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
      J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35984 / RP62A.

    Entry informationi

    Entry nameiDAGK_STAEQ
    AccessioniPrimary (citable) accession number: Q5HN36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 13, 2009
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3