Q5HMC7 (TENA_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative thiaminase-2
EC=3.5.99.2

Alternative name(s):
Thiaminase II

Gene names

Name:	tenA
Ordered Locus Names:	SERP1701

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Thiamine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole.
Pathway	Cofactor metabolism; thiamine degradation; 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole from thiamine: step 1/1.
Sequence similarities	Belongs to the thiaminase-2 family.

Ontologies

Keywords
Biological process	Thiamine catabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	thiaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Putative thiaminase-2		PRO_0000293616
Sites
	Active site	137	1	Nucleophile By similarity
	Binding site	44	1	Thiamine By similarity
	Binding site	141	1	Thiamine By similarity
	Binding site	167	1	Thiamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HMC7 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: C520C51829A236B2	FASTA	229	26,900
	10 20 30 40 50 60 MTFSKELREA SRPIIDDIYN DGFIQDLLAG KLSNQAVRQY LRADASYLKE FTNIYAMLIP 70 80 90 100 110 120 KMSSMEDVKF LVEQIEFMLE GEVEAHEVLA DFINEPYEEI VKEKVWPPSG DHYIKHMYFN 130 140 150 160 170 180 AFARENAAFT IAAMAPCPYV YAVIGKRAME DPKLNKESVT SKWFQFYSTE MDELVDVFDQ 190 200 210 220 LMDRLTKHCS ETEKKEIKEN FLQSTIHERH FFNMAYINEK WEYGGNNNE

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW55079.1.
RefSeq	YP_189266.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HMC7.
ModBase	Search...
Protein-protein interaction databases
STRING	176279.SERP1701.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW55079; AAW55079; SERP1701.
GeneID	3242123.
KEGG	ser:SERP1701.
PATRIC	19614325. VBIStaEpi130894_1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0819.
HOGENOM	HOG000280169.
KO	K03707.
OMA	LLPCPWT.
ProtClustDB	CLSK885763.
Enzyme and pathway databases
BioCyc	SEPI176279:GJJB-1770-MONOMER.
UniPathway	UPA00841; UER00805.
Family and domain databases
Gene3D	1.20.910.10. 1 hit.
InterPro	IPR016084. Haem_Oase-like_multi-hlx. IPR004305. Thiaminase-2/PQQC. [Graphical view]
Pfam	PF03070. TENA_THI-4. 1 hit. [Graphical view]
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TENA_STAEQ

Accession

Primary (citable) accession number: Q5HMC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program