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Protein

Aminopyrimidine aminohydrolase

Gene

tenA

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an amino-pyrimidine hydrolysis reaction at the C5' of the pyrimidine moiety of thiamine compounds, a reaction that is part of a thiamine salvage pathway. Thus, catalyzes the conversion of 4-amino-5-aminomethyl-2-methylpyrimidine to 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP). Is also able to catalyze the hydrolytic cleavage of thiamine; however, this thiaminase activity may not be physiologically relevant. Therefore, is probably involved in the regeneration of the thiamine pyrimidine from thiamine degraded products present in the environment, rather than in thiamine degradation.By similarity

Catalytic activityi

4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia.By similarity
Thiamine + H2O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole.By similarity

Pathway:ithiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441SubtrateBy similarity
Sitei47 – 471Increases nucleophilicity of active site CysBy similarity
Active sitei137 – 1371NucleophileBy similarity
Binding sitei141 – 1411SubtrateBy similarity
Binding sitei167 – 1671SubtrateBy similarity
Active sitei208 – 2081Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Thiamine biosynthesis

Enzyme and pathway databases

BioCyciSEPI176279:GJJB-1770-MONOMER.
UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopyrimidine aminohydrolaseBy similarity (EC:3.5.99.2By similarity)
Alternative name(s):
Thiaminase IIBy similarity
Gene namesi
Name:tenA
Ordered Locus Names:SERP1701
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Aminopyrimidine aminohydrolasePRO_0000293616Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi176279.SERP1701.

Structurei

3D structure databases

ProteinModelPortaliQ5HMC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TenA family.Curated

Phylogenomic databases

eggNOGiCOG0819.
HOGENOMiHOG000280169.
KOiK03707.
OMAiLLPCPWT.
OrthoDBiEOG6JMMXP.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
InterProiIPR016084. Haem_Oase-like_multi-hlx.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamiPF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
TIGRFAMsiTIGR04306. salvage_TenA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5HMC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFSKELREA SRPIIDDIYN DGFIQDLLAG KLSNQAVRQY LRADASYLKE
60 70 80 90 100
FTNIYAMLIP KMSSMEDVKF LVEQIEFMLE GEVEAHEVLA DFINEPYEEI
110 120 130 140 150
VKEKVWPPSG DHYIKHMYFN AFARENAAFT IAAMAPCPYV YAVIGKRAME
160 170 180 190 200
DPKLNKESVT SKWFQFYSTE MDELVDVFDQ LMDRLTKHCS ETEKKEIKEN
210 220
FLQSTIHERH FFNMAYINEK WEYGGNNNE
Length:229
Mass (Da):26,900
Last modified:February 15, 2005 - v1
Checksum:iC520C51829A236B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW55079.1.
RefSeqiWP_001829894.1. NC_002976.3.

Genome annotation databases

EnsemblBacteriaiAAW55079; AAW55079; SERP1701.
KEGGiser:SERP1701.
PATRICi19614325. VBIStaEpi130894_1661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW55079.1.
RefSeqiWP_001829894.1. NC_002976.3.

3D structure databases

ProteinModelPortaliQ5HMC7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176279.SERP1701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW55079; AAW55079; SERP1701.
KEGGiser:SERP1701.
PATRICi19614325. VBIStaEpi130894_1661.

Phylogenomic databases

eggNOGiCOG0819.
HOGENOMiHOG000280169.
KOiK03707.
OMAiLLPCPWT.
OrthoDBiEOG6JMMXP.

Enzyme and pathway databases

UniPathwayiUPA00060.
BioCyciSEPI176279:GJJB-1770-MONOMER.

Family and domain databases

Gene3Di1.20.910.10. 1 hit.
InterProiIPR016084. Haem_Oase-like_multi-hlx.
IPR004305. Thiaminase-2/PQQC.
IPR027574. Thiaminase_II.
[Graphical view]
PfamiPF03070. TENA_THI-4. 1 hit.
[Graphical view]
SUPFAMiSSF48613. SSF48613. 1 hit.
TIGRFAMsiTIGR04306. salvage_TenA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35984 / RP62A.

Entry informationi

Entry nameiTENA_STAEQ
AccessioniPrimary (citable) accession number: Q5HMC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: February 15, 2005
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.