Q5HM56 (ISDG_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase isdG
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant isdG
Iron-responsive surface determinant isdG

Gene names

Name:	isdG
Ordered Locus Names:	SERP1773

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP MF_01272
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. HAMAP MF_01272
Subunit structure	Homodimer By similarity. HAMAP MF_01272
Subcellular location	Cytoplasm By similarity HAMAP MF_01272.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 104	104	Heme-degrading monooxygenase isdG HAMAP MF_01272		PRO_0000270098
Sites
	Metal binding	6	1	Iron Potential
	Metal binding	76	1	Iron (heme axial ligand) Potential
	Site	66	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5HM56 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: D4D94713D8EDF66F	FASTA	104	12,023
	10 20 30 40 50 60 MFVVTNRITV KKGYAKQMAP NFTKGGPIES LKGFEGIEVW QIDKDDYSED MYVNSWWETE 70 80 90 100 EDFKNWVNSD VFKQAHKNTG KSEDSPVIKS EIVKSNVLSS LNRR

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW55132.1.
RefSeq	YP_189337.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HM56.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HM56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000041472; EBSTAP00000040015; EBSTAG00000041470.
GeneID	3241230.
GenomeReviews	Gene locus SERP1773 in contig CP000029_GR.
KEGG	ser:SERP1773.
PATRIC	19614479. VBIStaEpi130894_1729.
TIGR	SERP1773.
Phylogenomic databases
eggNOG	COG2329.
GeneTree	EBGT00050000024362.
HOGENOM	HBG616589.
OMA	GFAEKMA.
ProtClustDB	PRK13315.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP1773-MONOMER.
Family and domain databases
HAMAP	MF_01272. IsdG. [Tree]
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
KO	K07145.
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISDG_STAEQ

Accession

Primary (citable) accession number: Q5HM56

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program