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Protein

PTS system lactose-specific EIICB component

Gene

lacE

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei486Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIICB componentBy similarity
Alternative name(s):
EIICB-LacBy similarity
Short name:
EII-LacBy similarity
Including the following 2 domains:
PTS system lactose-specific EIIC componentBy similarity
Alternative name(s):
Lactose permease IIC componentBy similarity
PTS system lactose-specific EIIB componentBy similarity (EC:2.7.1.207By similarity)
Alternative name(s):
Lactose-specific phosphotransferase enzyme IIB componentBy similarity
Gene namesi
Name:lacEBy similarity
Ordered Locus Names:SERP1790
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000531 Componenti: Chromosome

Subcellular locationi

Q5HM40:
  • Cell membrane PROSITE-ProRule annotationBy similarity; Multi-pass membrane protein PROSITE-ProRule annotationBy similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei64 – 84HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei103 – 123HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei137 – 157HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei176 – 196HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei222 – 242HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei339 – 359HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865931 – 582PTS system lactose-specific EIICB componentAdd BLAST582

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei486Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5HM40.

Expressioni

Inductioni

Induced by lactose, galactose and galactose-6-P. Repressed by glucose.By similarity

Interactioni

Protein-protein interaction databases

STRINGi176279.SERP1790.

Structurei

3D structure databases

ProteinModelPortaliQ5HM40.
SMRiQ5HM40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 409PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST402
Domaini479 – 582PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST104

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000235576.
KOiK02787.
K02788.
OMAiISAMPFM.

Family and domain databases

InterProiView protein in InterPro
IPR036095. PTS_EIIB-like_sf.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004801. PTS_IIC_lac_spec.
PfamiView protein in Pfam
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00394. lac_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
TIGR00853. pts-lac. 1 hit.
PROSITEiView protein in PROSITE
PS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5HM40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLIAWIEK GKPFFEKISR NIYLRAIRDG FIAAIPIILF SSIFILITYV
60 70 80 90 100
PNVFGFTWSK TMEGILMKPY NYTMGIVGLL VAGTTAKSLT DSYNRKLDKT
110 120 130 140 150
NQINFISTMM AAICGFLFLA ADPVKDGGFS SAFMGTKGLL TAFISAFITV
160 170 180 190 200
IVYNFFVKRN ITIKMPKEVP PNISQVFKDI FPLSAVILIL YALDLLSRAI
210 220 230 240 250
VHTNVANAVL KVFEPLFTAA DGWIGVTLIF GAFAFFWFVG IHGPSIVEPA
260 270 280 290 300
IAAITYANLE TNLHLIQAGE HADKVITPGT QMFVATMGGT GATLVVPFMF
310 320 330 340 350
MWLTKSKRNK AIGRASVVPT FFGVNEPILF GAPLVLNPVF FIPFIFAPIV
360 370 380 390 400
NIWIFKFFVD VLNMNSFSIF LPWTTPGPLG IVMGTGFAFW SFVLAILLIV
410 420 430 440 450
VDVIIYYPFL KVYDEQVLEE ELGNKEANNE LKEKVSANFD TKKADAILAT
460 470 480 490 500
AGASEADTDD TSSVDETTST SSTDTISEQT NVLVLCAGGG TSGLLANALN
510 520 530 540 550
KAAEEYEVPV KAAAGGYGAH MDIMKDYQLI ILAPQVASNF EDIKQDTDRL
560 570 580
GIKLAKTEGA QYIKLTRDGE AALEFVKQQF NN
Length:582
Mass (Da):63,765
Last modified:February 15, 2005 - v1
Checksum:i256AC4A2AC536C1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW55166.1.
RefSeqiWP_001829731.1. NC_002976.3.

Genome annotation databases

EnsemblBacteriaiAAW55166; AAW55166; SERP1790.
KEGGiser:SERP1790.

Similar proteinsi

Entry informationi

Entry nameiPTLCB_STAEQ
AccessioniPrimary (citable) accession number: Q5HM40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: October 25, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome