Reviewed, UniProtKB/Swiss-Prot Q5HL73 (PTG3C_STAEQ)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICBA component
Alternative name(s):
    EIICBA-Glc
      Short name=EII-Glc
Including the following 3 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
    3- Recommended name:
            Glucose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Ordered Locus Names:	SERP2114

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	PTS system glucose-specific EIICBA component		PRO_0000351403
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	59 – 79	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	328 – 348	21	Potential
	Transmembrane	355 – 375	21	Potential
	Transmembrane	378 – 398	21	Potential
	Domain	3 – 414	412	PTS EIIC type-1
	Domain	425 – 506	82	PTS EIIB type-1
	Domain	547 – 651	105	PTS EIIA type-1
Sites
	Active site	447	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	599	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HL73-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: AD6EC55F6C15C4C2	FASTA	675	73,359
	10 20 30 40 50 60 MFKKLFGQMQ RIGKALMLPV AILPAAGLLL AIGTAFQGEA LQHYLPFIKN DIVQQIANML 70 80 90 100 110 120 TGAGGIIFDN LPIIFALGVA IGLAGGDGVA AIAAFVGFII LNKTMGAFLH VTPDKLSDPT 130 140 150 160 170 180 NGYANVLGIP TLQTGVFGGI IIGALAAWCY NKFYNITLPS YLGFFAGKRF VPIMMATTSF 190 200 210 220 230 240 ILAFPMAIIW PTIQNGLNAF SEGLLDSNTG LAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 250 260 270 280 290 300 GSWKNAAGEI IRGDQRIFIE QIREGAHLTS GKFMQGEFPV MMFGLPAAAL AIYQTAKPEN 310 320 330 340 350 360 KKVVAGLMIS AALTSFLTGI TEPLEFSFLF VAPFLFVIHA VLDGLSFLTL YLLNVHLGYT 370 380 390 400 410 420 FSGGFIDYVL LGILPNKTAW WLVIPVGIIY AVIYYFVFRF LIVKFNYKTP GREDKKSSVT 430 440 450 460 470 480 TTSASQLPFD VLKAMGGKEN IKHLDACITR LRVEVNEKSK VDVAGLKSLG ASGVLEVGNN 490 500 510 520 530 540 MQAIFGPKSD QIKHDMAKII SGEITKPSET TIDEEVSDDP VHVEDIVETE IYAPGHGEII 550 560 570 580 590 600 PLSEVPDKVF SEKMMGDGIG FVPDSGEIVA PFDGTVKTIF PTKHAIGLES DSGVEVLIHI 610 620 630 640 650 660 GIDTVKLNGE GFESLVNTDE PVTQGQPLMK IDLEYLKEHA PSIITPVIIT NQEDKTLTIE 670 DVKQVDPGKA IMTIK

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW52991.1.
RefSeq	YP_189670.1.
3D structure databases
HSSP	HSSP built from PDB template 1O2F based on UniProtKB P69786.
SMR	Q5HL73. Positions 431-500, 529-675.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HL73.
Genome annotation databases
GeneID	3242483.
GenomeReviews	Gene locus SERP2114 in contig CP000029_GR.
KEGG	ser:SERP2114.
TIGR	SERP2114.
Phylogenomic databases
eggNOG	COG1263.
HOGENOM	HBG571563.
OMA	CIPAIAL.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2114-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAEQ

Accession

Primary (citable) accession number: Q5HL73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)