PTS system glucose-specific EIICBA component - Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

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Q5HL73 (PTG3C_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
PTS system glucose-specific EIICBA component

Alternative name(s):
EIICBA-Glc
Short name=EII-Glc

Including the following 3 domains:

Glucose permease IIC component
Alternative name(s):
PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component
EC=2.7.1.69
Alternative name(s):
PTS system glucose-specific EIIB component
Glucose-specific phosphotransferase enzyme IIA component
EC=2.7.1.-
Alternative name(s):
PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Ordered Locus Names:	SERP2114

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 675

675

PTS system glucose-specific EIICBA component

PRO_0000351403

Regions

Transmembrane

16 – 36

Helical; Potential

Transmembrane

59 – 79

Helical; Potential

Transmembrane

81 – 101

Helical; Potential

Transmembrane

126 – 146

Helical; Potential

Transmembrane

170 – 190

Helical; Potential

Transmembrane

211 – 231

Helical; Potential

Transmembrane

273 – 293

Helical; Potential

Transmembrane

303 – 323

Helical; Potential

Transmembrane

328 – 348

Helical; Potential

Transmembrane

355 – 375

Helical; Potential

Transmembrane

378 – 398

Helical; Potential

Domain

3 – 414

412

PTS EIIC type-1

Domain

425 – 506

PTS EIIB type-1

Domain

547 – 651

105

PTS EIIA type-1

Sites

Active site

447

Phosphocysteine intermediate; for EIIB activity By similarity

Active site

599

Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q5HL73 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: AD6EC55F6C15C4C2
Show »

FASTA

675

73,359

        10         20         30         40         50         60 
MFKKLFGQMQ RIGKALMLPV AILPAAGLLL AIGTAFQGEA LQHYLPFIKN DIVQQIANML 

        70         80         90        100        110        120 
TGAGGIIFDN LPIIFALGVA IGLAGGDGVA AIAAFVGFII LNKTMGAFLH VTPDKLSDPT 

       130        140        150        160        170        180 
NGYANVLGIP TLQTGVFGGI IIGALAAWCY NKFYNITLPS YLGFFAGKRF VPIMMATTSF 

       190        200        210        220        230        240 
ILAFPMAIIW PTIQNGLNAF SEGLLDSNTG LAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 

       250        260        270        280        290        300 
GSWKNAAGEI IRGDQRIFIE QIREGAHLTS GKFMQGEFPV MMFGLPAAAL AIYQTAKPEN 

       310        320        330        340        350        360 
KKVVAGLMIS AALTSFLTGI TEPLEFSFLF VAPFLFVIHA VLDGLSFLTL YLLNVHLGYT 

       370        380        390        400        410        420 
FSGGFIDYVL LGILPNKTAW WLVIPVGIIY AVIYYFVFRF LIVKFNYKTP GREDKKSSVT 

       430        440        450        460        470        480 
TTSASQLPFD VLKAMGGKEN IKHLDACITR LRVEVNEKSK VDVAGLKSLG ASGVLEVGNN 

       490        500        510        520        530        540 
MQAIFGPKSD QIKHDMAKII SGEITKPSET TIDEEVSDDP VHVEDIVETE IYAPGHGEII 

       550        560        570        580        590        600 
PLSEVPDKVF SEKMMGDGIG FVPDSGEIVA PFDGTVKTIF PTKHAIGLES DSGVEVLIHI 

       610        620        630        640        650        660 
GIDTVKLNGE GFESLVNTDE PVTQGQPLMK IDLEYLKEHA PSIITPVIIT NQEDKTLTIE 

       670 
DVKQVDPGKA IMTIK

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References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.

Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW52991.1.
RefSeq	YP_189670.1. NC_002976.3.
3D structure databases
HSSP	HSSP built from PDB template 1O2F based on UniProtKB P69786.
ProteinModelPortal	Q5HL73.
SMR	Q5HL73. Positions 431-500.
ModBase	Search...
Protein-protein interaction databases
STRING	176279.SERP2114.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW52991; AAW52991; SERP2114.
GeneID	3242483.
KEGG	ser:SERP2114.
PATRIC	19615136. VBIStaEpi130894_2057.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1263.
HOGENOM	HOG000250993.
KO	K02763. K02764. K02765.
OMA	MESAGQI.
ProtClustDB	CLSK872840.
Enzyme and pathway databases
BioCyc	SEPI176279:GJJB-2191-MONOMER.
Family and domain databases
Gene3D	3.30.1360.60. 1 hit.
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAEQ

Accession

Primary (citable) accession number: Q5HL73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Including the following 3 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents