Reviewed, UniProtKB/Swiss-Prot Q5HL59 (ROCA_STAEQ)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	SERP2128

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056521
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HL59-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 99EE786D43629FF4	FASTA	514	56,876
	10 20 30 40 50 60 MVVPFKNEPG IDFSVQTNVE RFNEELRKVK AQLGQDIPLV INGEKLTKTD TFNSVNPANT 70 80 90 100 110 120 SQLIAKVSKA TQDDIEKAFE SANHAYQSWK KWSHKDRAEL LLRVAAIIRR RKEEISAIMV 130 140 150 160 170 180 YEAGKPWDEA VGDAAEGIDF IEYYARSMME LADGKPVLDR EGEHNRYFYK PIGTGVTIPP 190 200 210 220 230 240 WNFPFAIMAG TTLAPVVAGN TVLLKPAEDT VLTAYKLMEI LEEAGLPQGV VNFVPGDPKE 250 260 270 280 290 300 IGDYLVDHKD THFVTFTGSR ATGTRIYERS AVVQEGQQFL KRVIAEMGGK DAIVVDNNVD 310 320 330 340 350 360 TDLAAEAIVT SAFGFSGQKC SACSRAIVHQ DVHDEILEKA IQLTQKLTLG NTEENTFMGP 370 380 390 400 410 420 VINQKQFDKI KNYIEIGKKE GKLETGGGTD DSTGYFIEPT IFSGLQSADR IMQEEIFGPV 430 440 450 460 470 480 VGFIKVKDFD EAIEVANDTD YGLTGAVITN HREHWIKAVN EFDVGNLYLN RGCTAAVVGY 490 500 510 HPFGGFKMSG TDAKTGSPDY LLNFLEQKVV SEMF

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53020.1.
RefSeq	YP_189684.1.
3D structure databases
SMR	Q5HL59. Positions 2-514.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HL59.
Genome annotation databases
GeneID	3241657.
GenomeReviews	Gene locus SERP2128 in contig CP000029_GR.
KEGG	ser:SERP2128.
TIGR	SERP2128.
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	SINPANT.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2128-MONOMER.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_STAEQ

Accession

Primary (citable) accession number: Q5HL59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)