Q5HL21 (ALF1_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-biphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	SERP2166

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 296	295	Fructose-bisphosphate aldolase class 1 HAMAP MF_00729		PRO_0000216911
Sites
	Active site	175	1	Proton acceptor By similarity
	Active site	212	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HL21 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 81FC6BCFD86EB241	FASTA	296	32,993
	10 20 30 40 50 60 MNKEQLEKMT HGKGFIAALD QSGGSTPKAL KEYGVNEDQY SNEDEMFQLV HDMRTRVVTS 70 80 90 100 110 120 PSFSPDKILG AILFEQTMDR EVEGKYTGDY LADKGVVPFL KVDKGLAEEK NGVQLMKPID 130 140 150 160 170 180 DLDATLNRAN ERHIFGTKMR SNILELNEQG IKDVVEQQFE FAKKIIAKGL VPIIEPEVNI 190 200 210 220 230 240 NAKDKSEIEK VLKAEIKKGL DSLNDDQLVM LKLTIPTEAN LYKDLADHPN VVRVVVLSGG 250 260 270 280 290 YSRDEANKLL KDNDELIASF SRALASDLRA SQSQEEFDKA LGDAVDSIYD ASVNKN

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW52952.1.
RefSeq	YP_189722.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HL21.
SMR	Q5HL21. Positions 1-294.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HL21.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000042290; EBSTAP00000040833; EBSTAG00000042288.
GeneID	3240908.
GenomeReviews	Gene locus SERP2166 in contig CP000029_GR.
KEGG	ser:SERP2166.
NMPDR	fig|176279.3.peg.2428.
PATRIC	19615240. VBIStaEpi130894_2109.
TIGR	SERP2166.
Phylogenomic databases
eggNOG	COG3588.
GeneTree	EBGT00050000024908.
HOGENOM	HBG307137.
OMA	GTKMRSV.
ProtClustDB	PRK05377.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2166-MONOMER.
Family and domain databases
HAMAP	MF_00729. FBP_aldolase_1. [Tree]
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. IPR023014. FBP_aldolase_I_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01623.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_STAEQ

Accession

Primary (citable) accession number: Q5HL21

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 46 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program