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Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSEPI176279:GJJB-2443-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SERP2365
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000531: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Pyruvate formate-lyase-activating enzymePRO_0000271717Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176279.SERP2365.

Structurei

3D structure databases

ProteinModelPortaliQ5HKI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiRFVLFMQ.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HKI0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE
60 70 80 90 100
VTVDEMVNEI LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV
110 120 130 140 150
HTCIDTSAGC VNDTPAFNRH FDELQKHTDL ILLDIKHIDN DKHIKLTGKP
160 170 180 190 200
NTHILKFARK LSDMKQPVWI RHVLVPGISD DKEDLIKLGE FINSLDNVEK
210 220 230 240 250
FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY VNFNGKIPVT

L
Length:251
Mass (Da):28,555
Last modified:February 15, 2005 - v1
Checksum:i90EC4BF0E80399E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW53178.1.
RefSeqiYP_189913.1. NC_002976.3.

Genome annotation databases

EnsemblBacteriaiAAW53178; AAW53178; SERP2365.
GeneIDi3240431.
KEGGiser:SERP2365.
PATRICi19615619. VBIStaEpi130894_2297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW53178.1.
RefSeqiYP_189913.1. NC_002976.3.

3D structure databases

ProteinModelPortaliQ5HKI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176279.SERP2365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW53178; AAW53178; SERP2365.
GeneIDi3240431.
KEGGiser:SERP2365.
PATRICi19615619. VBIStaEpi130894_2297.

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011458.
KOiK04069.
OMAiRFVLFMQ.
OrthoDBiEOG64FKHC.

Enzyme and pathway databases

BioCyciSEPI176279:GJJB-2443-MONOMER.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35984 / RP62A.

Entry informationi

Entry nameiPFLA_STAEQ
AccessioniPrimary (citable) accession number: Q5HKI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: February 15, 2005
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.