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Q5HKI0 (PFLA_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase-activating enzyme

Short name=PFL-activating enzyme
EC=1.97.1.4
Gene names
Name:pflA
Ordered Locus Names:SERP2365
OrganismStaphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]
Taxonomic identifier176279 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Pyruvate formate-lyase-activating enzyme
PRO_0000271717

Sites

Metal binding291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HKI0 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 90EC4BF0E80399E3

FASTA25128,555
        10         20         30         40         50         60 
MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE VTVDEMVNEI 

        70         80         90        100        110        120 
LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV HTCIDTSAGC VNDTPAFNRH 

       130        140        150        160        170        180 
FDELQKHTDL ILLDIKHIDN DKHIKLTGKP NTHILKFARK LSDMKQPVWI RHVLVPGISD 

       190        200        210        220        230        240 
DKEDLIKLGE FINSLDNVEK FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY 

       250 
VNFNGKIPVT L 

« Hide

References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000029 Genomic DNA. Translation: AAW53178.1.
RefSeqYP_189913.1. NC_002976.3.

3D structure databases

ProteinModelPortalQ5HKI0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176279.SERP2365.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW53178; AAW53178; SERP2365.
GeneID3240431.
KEGGser:SERP2365.
PATRIC19615619. VBIStaEpi130894_2297.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011458.
KOK04069.
OMAWEMETNK.
OrthoDBEOG64FKHC.

Enzyme and pathway databases

BioCycSEPI176279:GJJB-2443-MONOMER.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_STAEQ
AccessionPrimary (citable) accession number: Q5HKI0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families