Q5HKG6 (BUTA_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacetyl reductase [(S)-acetoin forming]
EC=1.1.1.304

Alternative name(s):
Acetoin(diacetyl) reductase
Short name=AR
Meso-2,3-butanediol dehydrogenase

Gene names

Name:	butA
Ordered Locus Names:	SERP2379

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH By similarity.
Catalytic activity	(S)-acetoin + NAD+ = diacetyl + NADH.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	acetoin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	diacetyl reductase ((S)-acetoin forming) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Diacetyl reductase [(S)-acetoin forming]		PRO_0000054546
Regions
	Nucleotide binding	6 – 30	25	NAD By similarity
Sites
	Active site	152	1	Proton acceptor By similarity
	Active site	156	1	By similarity
	Binding site	139	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HKG6 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 7987749B2E0CA1E6	FASTA	257	27,917
	10 20 30 40 50 60 MSKTAIITGS AGGLGKGIAE RLANDGFNIV LQDINEALLL ETEKEFKEKG YQAVAFKSDV 70 80 90 100 110 120 SKKKEQEELV QFAVTEFGQL DVMVNNAGVD AVTPILEIGE EELSKLFNIN VFGTLFGIQA 130 140 150 160 170 180 AANQFIKQKS KGKIINACSI AGHESYEVLG TYSATKHSVR SFTQTAAKEL ADKGITVNAY 190 200 210 220 230 240 CPGVAKTEMW DRIDEEMVKL DDSLEIGDAF EAFSSEIKLG RYQEPSDVAN LVSFLASNDS 250 DYITGQSILT DGGLVYR

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53206.1.
RefSeq	YP_189927.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HKG6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HKG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000043174; EBSTAP00000041717; EBSTAG00000043172.
GeneID	3240936.
GenomeReviews	Gene locus SERP2379 in contig CP000029_GR.
KEGG	ser:SERP2379.
NMPDR	fig|176279.3.peg.2029.
PATRIC	19615645. VBIStaEpi130894_2310.
TIGR	SERP2379.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	EBGT00050000023864.
HOGENOM	HBG750976.
OMA	AGHESYE.
PhylomeDB	Q5HKG6.
ProtClustDB	CLSK580723.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2379-MONOMER.
Family and domain databases
InterPro	IPR014007. 23BDH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K03366.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR02415. 23BDH. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUTA_STAEQ

Accession

Primary (citable) accession number: Q5HKG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program