Reviewed, UniProtKB/Swiss-Prot Q5HKB7 (ARSC2_STAEQ)

Names and origin

Protein names

Recommended name:
    Protein arsC 2
Alternative name(s):
    Arsenate reductase 2
    EC=1.20.4.-
    Arsenical pump modifier 2
    Low molecular weight protein-tyrosine-phosphatase 2
    EC=3.1.3.48

Gene names

Name:	arsC2
Ordered Locus Names:	SERP2431

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity. HAMAP MF_01624
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity. HAMAP MF_01624
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Protein arsC 2 HAMAP MF_01624		PRO_0000162529
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	82	1	Nucleophile; for reductase activity By similarity
	Active site	89	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 82		Redox-active; alternate By similarity
	Disulfide bond	82 ↔ 89		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HKB7-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 405C72DCE141F55C	FASTA	131	14,693
	10 20 30 40 50 60 MDKKTIYFIC TGNSCRSQMA EGWGKKILGD EWQVYSGGIE AHGVNPKAIE AMKEVGIDIS 70 80 90 100 110 120 NHTSNLIDQN ILHQSDLVVT LCSDADKNCP ILPPSVKKEH WGFDDPAGKP WSEFQRVRDE 130 IKTAIESFKT R

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53288.1.
RefSeq	YP_189976.1.
3D structure databases
SMR	Q5HKB7. Positions 2-131.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HKB7.
Genome annotation databases
GeneID	3242360.
GenomeReviews	Gene locus SERP2431 in contig CP000029_GR.
KEGG	ser:SERP2431.
TIGR	SERP2431.
Phylogenomic databases
eggNOG	COG0394.
HOGENOM	HBG730979.
OMA	EMRIVIT.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2431-MONOMER.
Family and domain databases
HAMAP	MF_01624. Arsenate_reduct. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC2_STAEQ

Accession

Primary (citable) accession number: Q5HKB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)