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Q5HK76 (HSDR_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-1 restriction enzyme R protein

EC=3.1.21.3
Alternative name(s):
Type I restriction enzyme R protein
Gene names
Name:hsdR
Ordered Locus Names:SERP2474
OrganismStaphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]
Taxonomic identifier176279 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length930 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Subunit R is required for both nuclease and ATPase activities, but not for modification By similarity.

Catalytic activity

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Subunit structure

The type I restriction/modification system is composed of three polypeptides R, M and S By similarity.

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases By similarity.

Sequence similarities

Belongs to the HsdR family.

Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Type I site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 930930Type-1 restriction enzyme R protein
PRO_0000077271

Regions

Domain254 – 418165Helicase ATP-binding
Nucleotide binding268 – 2747ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HK76 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: AFE422D1D08A0A1D

FASTA930109,659
        10         20         30         40         50         60 
MVYQSEYALE NEVLQQLENL GYERVNIHNV EQLHDNFRTI INERHKEKLN GKPLTDREFE 

        70         80         90        100        110        120 
RLMTGINGKS VFDSAMQLRD SYVLKRDDDT DLYLNFMNLK QWCQNKFQVT NQISVKDKYK 

       130        140        150        160        170        180 
SRYDVTILIN GLPLVQIELK RSGVAITEAF NQIERYRRQN YTGLFRYIQL FVVSNKMETR 

       190        200        210        220        230        240 
YYANSDREIF KGQMFYWSNE QNERINYLKD FIEDFLESCH ISKMISRYMV VNETDKFLMA 

       250        260        270        280        290        300 
LRPYQVYAVE AILNRALETN NNGYIWHTTG SGKTLTSFKA SQLLSQEENI KKVIFLVDRK 

       310        320        330        340        350        360 
DLDNQTLAEF NKFQEDSVDF TDNTRKLLKQ LADPTLPLIV TTIQKMANAV KSNHSVMESY 

       370        380        390        400        410        420 
KQDKVIFIID ECHRTQFGDM HRLIKQHFEN AQYFGFTGTP RFEENKSQDG RATADIFDKC 

       430        440        450        460        470        480 
LHHYLIKDAI RDHNVLGFSV EYNQTFNSHE DLDEEYISKI NTSEIWMADE RIEAVCRHLI 

       490        500        510        520        530        540 
SNYHKKTDNG NYTSMFAVQS IPMAIKYYDT FQRLKNEGVH DLNVATIFTY QANEDAQEDE 

       550        560        570        580        590        600 
NHVHSREVLD RIMNDYNQTF KTNYNTDNFE GYFSDVSKRM KEVVRDDKID ILIVVNMFLT 

       610        620        630        640        650        660 
GFDSKKLNTL YVDKNLKHHD LIQAYSRTNR VEKERKPYGN IVCYRDLKKQ TDEAIEIFSQ 

       670        680        690        700        710        720 
TDNTDTVLSL SYEEYLDNFK DILQDVFQLA PTPLDVDKLE AEDLKKEFVI SFRDLSNTLI 

       730        740        750        760        770        780 
KLKTFDEFQF TEDELGIAEQ TYEDYKGKYL NIYEEVMRGK KVDGEEGVSV LDDIDFQVEL 

       790        800        810        820        830        840 
MRNDLINVKY IMDLIGQINL SDAKARDEKR HQIHKLLDKA DDQQLRLKAD LIRSFLDKVV 

       850        860        870        880        890        900 
PSLKEDSDIN EAYYEFEDKE KTKEIDAFAE QKAFSAMLLN EAVNEYEYSG NIDRKSIGQN 

       910        920        930 
ISEPFMKRKR KTDQIIQFIE DTVEKYGVVE 

« Hide

References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000029 Genomic DNA. Translation: AAW53360.1.
RefSeqYP_190017.1. NC_002976.3.

3D structure databases

ProteinModelPortalQ5HK76.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176279.SERP2474.

Protein family/group databases

REBASE10764. SepRPORF2472P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW53360; AAW53360; SERP2474.
GeneID3242316.
KEGGser:SERP2474.
PATRIC19615821. VBIStaEpi130894_2398.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0610.
HOGENOMHOG000003518.
KOK01153.
OMARFVETWL.
OrthoDBEOG6JTCB4.
ProtClustDBCLSK884469.

Enzyme and pathway databases

BioCycSEPI176279:GJJB-2552-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00348. hsdR. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHSDR_STAEQ
AccessionPrimary (citable) accession number: Q5HK76
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: February 15, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families