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Q5HK76

- HSDR_STAEQ

UniProt

Q5HK76 - HSDR_STAEQ

Protein

Type-1 restriction enzyme R protein

Gene

hsdR

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (15 Feb 2005)
      Previous versions | rss
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    Functioni

    Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi268 – 2747ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. Type I site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSEPI176279:GJJB-2552-MONOMER.

    Protein family/group databases

    REBASEi10764. SepRPORF2472P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-1 restriction enzyme R protein (EC:3.1.21.3)
    Alternative name(s):
    Type I restriction enzyme R protein
    Gene namesi
    Name:hsdR
    Ordered Locus Names:SERP2474
    OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
    Taxonomic identifieri176279 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000531: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Type-1 restriction enzyme R proteinPRO_0000077271Add
    BLAST

    Interactioni

    Subunit structurei

    The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

    Protein-protein interaction databases

    STRINGi176279.SERP2474.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5HK76.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini254 – 418165Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HsdR family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0610.
    HOGENOMiHOG000003518.
    KOiK01153.
    OMAiNNGYIWH.
    OrthoDBiEOG6JTCB4.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    IPR022625. TypeI_RM_Rsu_C.
    [Graphical view]
    PfamiPF12008. EcoR124_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    TIGRFAMsiTIGR00348. hsdR. 1 hit.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5HK76-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVYQSEYALE NEVLQQLENL GYERVNIHNV EQLHDNFRTI INERHKEKLN    50
    GKPLTDREFE RLMTGINGKS VFDSAMQLRD SYVLKRDDDT DLYLNFMNLK 100
    QWCQNKFQVT NQISVKDKYK SRYDVTILIN GLPLVQIELK RSGVAITEAF 150
    NQIERYRRQN YTGLFRYIQL FVVSNKMETR YYANSDREIF KGQMFYWSNE 200
    QNERINYLKD FIEDFLESCH ISKMISRYMV VNETDKFLMA LRPYQVYAVE 250
    AILNRALETN NNGYIWHTTG SGKTLTSFKA SQLLSQEENI KKVIFLVDRK 300
    DLDNQTLAEF NKFQEDSVDF TDNTRKLLKQ LADPTLPLIV TTIQKMANAV 350
    KSNHSVMESY KQDKVIFIID ECHRTQFGDM HRLIKQHFEN AQYFGFTGTP 400
    RFEENKSQDG RATADIFDKC LHHYLIKDAI RDHNVLGFSV EYNQTFNSHE 450
    DLDEEYISKI NTSEIWMADE RIEAVCRHLI SNYHKKTDNG NYTSMFAVQS 500
    IPMAIKYYDT FQRLKNEGVH DLNVATIFTY QANEDAQEDE NHVHSREVLD 550
    RIMNDYNQTF KTNYNTDNFE GYFSDVSKRM KEVVRDDKID ILIVVNMFLT 600
    GFDSKKLNTL YVDKNLKHHD LIQAYSRTNR VEKERKPYGN IVCYRDLKKQ 650
    TDEAIEIFSQ TDNTDTVLSL SYEEYLDNFK DILQDVFQLA PTPLDVDKLE 700
    AEDLKKEFVI SFRDLSNTLI KLKTFDEFQF TEDELGIAEQ TYEDYKGKYL 750
    NIYEEVMRGK KVDGEEGVSV LDDIDFQVEL MRNDLINVKY IMDLIGQINL 800
    SDAKARDEKR HQIHKLLDKA DDQQLRLKAD LIRSFLDKVV PSLKEDSDIN 850
    EAYYEFEDKE KTKEIDAFAE QKAFSAMLLN EAVNEYEYSG NIDRKSIGQN 900
    ISEPFMKRKR KTDQIIQFIE DTVEKYGVVE 930
    Length:930
    Mass (Da):109,659
    Last modified:February 15, 2005 - v1
    Checksum:iAFE422D1D08A0A1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW53360.1.
    RefSeqiWP_002489617.1. NC_002976.3.
    YP_190017.1. NC_002976.3.

    Genome annotation databases

    EnsemblBacteriaiAAW53360; AAW53360; SERP2474.
    GeneIDi3242316.
    KEGGiser:SERP2474.
    PATRICi19615821. VBIStaEpi130894_2398.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000029 Genomic DNA. Translation: AAW53360.1 .
    RefSeqi WP_002489617.1. NC_002976.3.
    YP_190017.1. NC_002976.3.

    3D structure databases

    ProteinModelPortali Q5HK76.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176279.SERP2474.

    Protein family/group databases

    REBASEi 10764. SepRPORF2472P.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAW53360 ; AAW53360 ; SERP2474 .
    GeneIDi 3242316.
    KEGGi ser:SERP2474.
    PATRICi 19615821. VBIStaEpi130894_2398.

    Phylogenomic databases

    eggNOGi COG0610.
    HOGENOMi HOG000003518.
    KOi K01153.
    OMAi NNGYIWH.
    OrthoDBi EOG6JTCB4.

    Enzyme and pathway databases

    BioCyci SEPI176279:GJJB-2552-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR006935. Helicase/UvrB_dom.
    IPR014001. Helicase_ATP-bd.
    IPR027417. P-loop_NTPase.
    IPR007409. Restrct_endonuc_type1_HsdR_N.
    IPR004473. Restrct_endonuc_typeI_HsdR.
    IPR022625. TypeI_RM_Rsu_C.
    [Graphical view ]
    Pfami PF12008. EcoR124_C. 1 hit.
    PF04313. HSDR_N. 1 hit.
    PF04851. ResIII. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    TIGRFAMsi TIGR00348. hsdR. 1 hit.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
      Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
      , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
      J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35984 / RP62A.

    Entry informationi

    Entry nameiHSDR_STAEQ
    AccessioniPrimary (citable) accession number: Q5HK76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: February 15, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3