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Protein

Type-1 restriction enzyme R protein

Gene

hsdR

Organism
Staphylococcus epidermidis (strain ATCC 35984 / RP62A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2747ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSEPI176279:GJJB-2552-MONOMER.

Protein family/group databases

REBASEi10764. SepRPORF2472P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme R protein (EC:3.1.21.3)
Alternative name(s):
Type I restriction enzyme R protein
Gene namesi
Name:hsdR
Ordered Locus Names:SERP2474
OrganismiStaphylococcus epidermidis (strain ATCC 35984 / RP62A)
Taxonomic identifieri176279 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000531 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Type-1 restriction enzyme R proteinPRO_0000077271Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

Protein-protein interaction databases

STRINGi176279.SERP2474.

Structurei

3D structure databases

ProteinModelPortaliQ5HK76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini254 – 418165Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HsdR family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0610.
HOGENOMiHOG000003518.
KOiK01153.
OMAiQLEKHNK.
OrthoDBiEOG6JTCB4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamiPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00348. hsdR. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HK76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYQSEYALE NEVLQQLENL GYERVNIHNV EQLHDNFRTI INERHKEKLN
60 70 80 90 100
GKPLTDREFE RLMTGINGKS VFDSAMQLRD SYVLKRDDDT DLYLNFMNLK
110 120 130 140 150
QWCQNKFQVT NQISVKDKYK SRYDVTILIN GLPLVQIELK RSGVAITEAF
160 170 180 190 200
NQIERYRRQN YTGLFRYIQL FVVSNKMETR YYANSDREIF KGQMFYWSNE
210 220 230 240 250
QNERINYLKD FIEDFLESCH ISKMISRYMV VNETDKFLMA LRPYQVYAVE
260 270 280 290 300
AILNRALETN NNGYIWHTTG SGKTLTSFKA SQLLSQEENI KKVIFLVDRK
310 320 330 340 350
DLDNQTLAEF NKFQEDSVDF TDNTRKLLKQ LADPTLPLIV TTIQKMANAV
360 370 380 390 400
KSNHSVMESY KQDKVIFIID ECHRTQFGDM HRLIKQHFEN AQYFGFTGTP
410 420 430 440 450
RFEENKSQDG RATADIFDKC LHHYLIKDAI RDHNVLGFSV EYNQTFNSHE
460 470 480 490 500
DLDEEYISKI NTSEIWMADE RIEAVCRHLI SNYHKKTDNG NYTSMFAVQS
510 520 530 540 550
IPMAIKYYDT FQRLKNEGVH DLNVATIFTY QANEDAQEDE NHVHSREVLD
560 570 580 590 600
RIMNDYNQTF KTNYNTDNFE GYFSDVSKRM KEVVRDDKID ILIVVNMFLT
610 620 630 640 650
GFDSKKLNTL YVDKNLKHHD LIQAYSRTNR VEKERKPYGN IVCYRDLKKQ
660 670 680 690 700
TDEAIEIFSQ TDNTDTVLSL SYEEYLDNFK DILQDVFQLA PTPLDVDKLE
710 720 730 740 750
AEDLKKEFVI SFRDLSNTLI KLKTFDEFQF TEDELGIAEQ TYEDYKGKYL
760 770 780 790 800
NIYEEVMRGK KVDGEEGVSV LDDIDFQVEL MRNDLINVKY IMDLIGQINL
810 820 830 840 850
SDAKARDEKR HQIHKLLDKA DDQQLRLKAD LIRSFLDKVV PSLKEDSDIN
860 870 880 890 900
EAYYEFEDKE KTKEIDAFAE QKAFSAMLLN EAVNEYEYSG NIDRKSIGQN
910 920 930
ISEPFMKRKR KTDQIIQFIE DTVEKYGVVE
Length:930
Mass (Da):109,659
Last modified:February 15, 2005 - v1
Checksum:iAFE422D1D08A0A1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW53360.1.
RefSeqiWP_002489617.1. NC_002976.3.

Genome annotation databases

EnsemblBacteriaiAAW53360; AAW53360; SERP2474.
KEGGiser:SERP2474.
PATRICi19615821. VBIStaEpi130894_2398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000029 Genomic DNA. Translation: AAW53360.1.
RefSeqiWP_002489617.1. NC_002976.3.

3D structure databases

ProteinModelPortaliQ5HK76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176279.SERP2474.

Protein family/group databases

REBASEi10764. SepRPORF2472P.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW53360; AAW53360; SERP2474.
KEGGiser:SERP2474.
PATRICi19615821. VBIStaEpi130894_2398.

Phylogenomic databases

eggNOGiCOG0610.
HOGENOMiHOG000003518.
KOiK01153.
OMAiQLEKHNK.
OrthoDBiEOG6JTCB4.

Enzyme and pathway databases

BioCyciSEPI176279:GJJB-2552-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamiPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00348. hsdR. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35984 / RP62A.

Entry informationi

Entry nameiHSDR_STAEQ
AccessioniPrimary (citable) accession number: Q5HK76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: February 15, 2005
Last modified: July 22, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.