Q5HK04 (GYRA_STAEQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	SERP2548

Organism

Staphylococcus epidermidis (strain ATCC 35984 / RP62A) [Complete proteome] [HAMAP]

Taxonomic identifier

176279 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 893	893	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145260
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HK04 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 2DC566AD0D2126CE	FASTA	893	100,144
	10 20 30 40 50 60 MAELPQSRIN ERNITSEMRE SFLDYAMSVI VSRALPDVRD GLKPVHRRIL YGLNEQGMTP 70 80 90 100 110 120 DKPYKKSARI VGDVMGKYHP HGDSSIYEAM VRMAQDFSYR YPLVDGQGNF GSMDGDGAAA 130 140 150 160 170 180 MRYTEARMTK ITLELLRDIN KDTIDFIDNY DGNEREPSVL PARFPNLLVN GAAGIAVGMA 190 200 210 220 230 240 TNIPPHNLTE VIDGVLSLSK NPDITINELM EDIQGPDFPT AGLVLGKSGI RRAYETGRGS 250 260 270 280 290 300 IQMRSRAEIE ERGGGRQRIV VTEIPFQVNK ARMIEKIAEL VRDKKIDGIT DLRDETSLRT 310 320 330 340 350 360 GVRVVIDVRK DANASVILNN LYKQTPLQTS FGVNMIALVN SRPKLINLKE ALIHYLEHQK 370 380 390 400 410 420 TVVRRRTEYN LKKARDRAHI LEGLRIALDH IDEIITTIRE SDTDKIAMAS LQERFKLTER 430 440 450 460 470 480 QAQAILDMRL RRLTGLERDK IESEYNELLE YIKELEEILA DEEVLLQLVR DELTEIKERF 490 500 510 520 530 540 GDERRTEIQL GGLEDLEDED LIPEEQIVIT LSHNNYIKRL PVSTYRSQNR GGRGIQGMNT 550 560 570 580 590 600 LDEDFVSQLV TMSTHDHVLF FTNKGRVYKL KGYEVPELSR QSKGIPIINA IELENDETIS 610 620 630 640 650 660 TMIAVKDLES EEDYLVFATK QGIVKRSSLS NFSRINKNGK IAINFKEDDE LIAVRLTTGN 670 680 690 700 710 720 EDILIGTAHA SLIRFSESTL RPLGRTAAGV KGISLREGDT VVGLDVADSE SEDEVLVVTE 730 740 750 760 770 780 NGYGKRTPVS EYRLSNRGGK GIKTATITER NGNIVCITTV TGEEDLMVVT NAGVIIRLDV 790 800 810 820 830 840 HDISQNGRAA QGVRLMKLGD GQFVSTVAKV NEEDDNEENA DEAQQSTTTE TADVEEVVDD 850 860 870 880 890 QTPGNAIHTE GDAEMESVES PENDDRIDIR QDFMDRVNED IESASDNEED SDE

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35984 / RP62A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000029 Genomic DNA. Translation: AAW53372.1.
RefSeq	YP_190089.1. NC_002976.3.
3D structure databases
ProteinModelPortal	Q5HK04.
SMR	Q5HK04. Positions 31-489.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HK04.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000041598; EBSTAP00000040141; EBSTAG00000041596.
GeneID	3242531.
GenomeReviews	Gene locus SERP2548 in contig CP000029_GR.
KEGG	ser:SERP2548.
NMPDR	fig|176279.3.peg.1682.
PATRIC	19615962. VBIStaEpi130894_2465.
TIGR	SERP2548.
Phylogenomic databases
eggNOG	COG0188.
GeneTree	EBGT00050000024676.
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	Q5HK04.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	SEPI176279:SERP2548-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STAEQ

Accession

Primary (citable) accession number: Q5HK04

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program