Q5HJP2 (BUTA_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacetyl reductase [(S)-acetoin forming]
EC=1.1.1.304

Alternative name(s):
Acetoin(diacetyl) reductase
Short name=AR
Meso-2,3-butanediol dehydrogenase

Gene names

Name:	butA
Ordered Locus Names:	SACOL0111

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH By similarity.
Catalytic activity	(S)-acetoin + NAD+ = diacetyl + NADH.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	acetoin catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	diacetyl reductase ((S)-acetoin forming) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Diacetyl reductase [(S)-acetoin forming]		PRO_0000054539
Regions
	Nucleotide binding	8 – 32	25	NAD By similarity
Sites
	Active site	154	1	Proton acceptor By similarity
	Active site	158	1	By similarity
	Binding site	141	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HJP2 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 114AFD0DC5D780CF	FASTA	258	27,216
	10 20 30 40 50 60 MTNNKVALVT GGAQGIGFKI AERLVEDGFK VAVVDFNEEG AKAAALKLSS DGTKAIAIKA 70 80 90 100 110 120 DVSNRDDVFN AVRQTAAQFG DFHVMVNNAG LGPTTPIDTI TEEQFKTVYG VNVAGVLWGI 130 140 150 160 170 180 QAAHEQFKKF NHGGKIINAT SQAGVEGNPG LSLYCSTKFA VRGLTQVAAQ DLASEGITVN 190 200 210 220 230 240 AFAPGIVQTP MMESIAVATA EEAGKPEAWG WEQFTSQIAL GRVSQPEDVS NVVSFLAGKD 250 SDYITGQTII VDGGMRFR

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW38754.1.
RefSeq	YP_185015.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HJP2.
SMR	Q5HJP2. Positions 4-257.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HJP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000010547; EBSTAP00000010282; EBSTAG00000010546.
GeneID	3236834.
GenomeReviews	Gene locus SACOL0111 in contig CP000046_GR.
KEGG	sac:SACOL0111.
PATRIC	19526478. VBIStaAur112458_0105.
TIGR	SACOL0111.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	EBGT00050000023864.
HOGENOM	HBG750976.
OMA	VAACVAY.
ProtClustDB	PRK08643.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL0111-MONOMER.
Family and domain databases
InterPro	IPR014007. 23BDH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K03366.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR02415. 23BDH. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUTA_STAAC

Accession

Primary (citable) accession number: Q5HJP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program