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Protein

Type-1 restriction enzyme R protein

Gene

hsdR

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Subunit R is required for both nuclease and ATPase activities, but not for modification.By similarity

Catalytic activityi

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2747ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-175-MONOMER.

Protein family/group databases

REBASEi10753. SauCOLORF180P.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-1 restriction enzyme R protein (EC:3.1.21.3)
Alternative name(s):
Type I restriction enzyme R protein
Gene namesi
Name:hsdR
Ordered Locus Names:SACOL0180
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Type-1 restriction enzyme R proteinPRO_0000077265Add
BLAST

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.By similarity

Protein-protein interaction databases

STRINGi93062.SACOL0180.

Structurei

3D structure databases

ProteinModelPortaliQ5HJH8.
SMRiQ5HJH8. Positions 2-730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini254 – 418165Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HsdR family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0610.
HOGENOMiHOG000003518.
KOiK01153.
OMAiPEKGRDK.
OrthoDBiEOG6JTCB4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamiPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00348. hsdR. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HJH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYQSEYALE NEMMNQLEQL GYERVTIRDN KQLLDNFRTI LNERHADKLE
60 70 80 90 100
GNPLTDKEFQ RLLTMIDGKS IFESARILRD KLPLRRDDES EIYLSFLDTK
110 120 130 140 150
SWCKNKFQVT NQVSVEDTYK ARYDVTILIN GLPLVQVELK RRGIDINEAF
160 170 180 190 200
NQVKRYRKQN YTGLFRYIQM FIISNGVETR YFSNNDSELL KSHMFYWSDK
210 220 230 240 250
QNNRINTLQS FAESFMRPCQ LAKMISRYMI INETDRILMA MRPYQVYAVE
260 270 280 290 300
ALIQQATETG NNGYVWHTTG SGKTLTSFKA SQILSQQDDI KKVIFLVDRK
310 320 330 340 350
DLDSQTEEEF NKFAKGAVDK TFNTSQLVRQ LNDKSLPLIV TTIQKMAKAI
360 370 380 390 400
QGNAPLLEQY KTNKVVFIID ECHRSQFGDM HRLVKQHFKN AQYFGFTGTP
410 420 430 440 450
RFPENSSQDG RTTADIFGRC LHTYLIRDAI HDGNVLGFSV DYINTFKNKA
460 470 480 490 500
LKAEDNSMVE AIDTEEVWLA DKRVELVTRH IINNHDKYTR NRQYSSIFTV
510 520 530 540 550
QSIHALIKYY ETFKRLNKKL EQPLTIAGIF TFKPNEDDRD GEVPYHSREK
560 570 580 590 600
LEIMISDYNK KFETNFSTDT TNEYFNHISK NVKKGVKDSK IDILIVVNMF
610 620 630 640 650
LTGFDSKVLN TLYVDKNLMY HDLIQAYSRT NRVEKESKPF GKIVNYRDLK
660 670 680 690 700
KETDDALRVF SQTNDTDTIL MRSYEEYKKE FMDAYRELKM IVPTPHMVDD
710 720 730 740 750
IQDEEELKRF VEAYRLLAKI ILRLKAFDEF EFTIDEIGMD EQENEDYKSK
760 770 780 790 800
YLAVYDQVKR ATAEKNKVSI LNDIDFEIEM MRNDTINVNY IMNILRQIDL
810 820 830 840 850
EDKAEQRRNQ EQIRRILDHA DDPTLRLKRD LIREFIDNVV PSLNKDDDID
860 870 880 890 900
QEYVNFESIK KEAEFKGFAG ERSIDEQALK TISNDYQYSG VVNPHHLKKM
910 920
IGDLPLKEKR KARKAIESFV AETTEKYGV
Length:929
Mass (Da):109,227
Last modified:February 15, 2005 - v1
Checksum:i0612EA7B026D3105
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW37476.1.
RefSeqiWP_000331347.1. NC_002951.2.
YP_185079.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW37476; AAW37476; SACOL0180.
GeneIDi23196015.
KEGGisac:SACOL0180.
PATRICi19526626. VBIStaAur112458_0179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW37476.1.
RefSeqiWP_000331347.1. NC_002951.2.
YP_185079.1. NC_002951.2.

3D structure databases

ProteinModelPortaliQ5HJH8.
SMRiQ5HJH8. Positions 2-730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0180.

Protein family/group databases

REBASEi10753. SauCOLORF180P.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW37476; AAW37476; SACOL0180.
GeneIDi23196015.
KEGGisac:SACOL0180.
PATRICi19526626. VBIStaAur112458_0179.

Phylogenomic databases

eggNOGiCOG0610.
HOGENOMiHOG000003518.
KOiK01153.
OMAiPEKGRDK.
OrthoDBiEOG6JTCB4.

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-175-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
IPR007409. Restrct_endonuc_type1_HsdR_N.
IPR004473. Restrct_endonuc_typeI_HsdR.
IPR022625. TypeI_RM_Rsu_C.
[Graphical view]
PfamiPF12008. EcoR124_C. 1 hit.
PF04313. HSDR_N. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00348. hsdR. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COL.

Entry informationi

Entry nameiHSDR_STAAC
AccessioniPrimary (citable) accession number: Q5HJH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: February 15, 2005
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.By similarity

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.