Q5HJH8 (HSDR_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-1 restriction enzyme R protein
EC=3.1.21.3

Alternative name(s):
Type I restriction enzyme R protein

Gene names

Name:	hsdR
Ordered Locus Names:	SACOL0180

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Subunit R is required for both nuclease and ATPase activities, but not for modification By similarity.
Catalytic activity	Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolyzed.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S By similarity.
Miscellaneous	Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases By similarity.
Sequence similarities	Belongs to the HsdR family. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	Restriction system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW Type I site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	Type-1 restriction enzyme R protein		PRO_0000077265
Regions
	Domain	254 – 418	165	Helicase ATP-binding
	Nucleotide binding	268 – 274	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HJH8 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 0612EA7B026D3105	FASTA	929	109,227
	10 20 30 40 50 60 MAYQSEYALE NEMMNQLEQL GYERVTIRDN KQLLDNFRTI LNERHADKLE GNPLTDKEFQ 70 80 90 100 110 120 RLLTMIDGKS IFESARILRD KLPLRRDDES EIYLSFLDTK SWCKNKFQVT NQVSVEDTYK 130 140 150 160 170 180 ARYDVTILIN GLPLVQVELK RRGIDINEAF NQVKRYRKQN YTGLFRYIQM FIISNGVETR 190 200 210 220 230 240 YFSNNDSELL KSHMFYWSDK QNNRINTLQS FAESFMRPCQ LAKMISRYMI INETDRILMA 250 260 270 280 290 300 MRPYQVYAVE ALIQQATETG NNGYVWHTTG SGKTLTSFKA SQILSQQDDI KKVIFLVDRK 310 320 330 340 350 360 DLDSQTEEEF NKFAKGAVDK TFNTSQLVRQ LNDKSLPLIV TTIQKMAKAI QGNAPLLEQY 370 380 390 400 410 420 KTNKVVFIID ECHRSQFGDM HRLVKQHFKN AQYFGFTGTP RFPENSSQDG RTTADIFGRC 430 440 450 460 470 480 LHTYLIRDAI HDGNVLGFSV DYINTFKNKA LKAEDNSMVE AIDTEEVWLA DKRVELVTRH 490 500 510 520 530 540 IINNHDKYTR NRQYSSIFTV QSIHALIKYY ETFKRLNKKL EQPLTIAGIF TFKPNEDDRD 550 560 570 580 590 600 GEVPYHSREK LEIMISDYNK KFETNFSTDT TNEYFNHISK NVKKGVKDSK IDILIVVNMF 610 620 630 640 650 660 LTGFDSKVLN TLYVDKNLMY HDLIQAYSRT NRVEKESKPF GKIVNYRDLK KETDDALRVF 670 680 690 700 710 720 SQTNDTDTIL MRSYEEYKKE FMDAYRELKM IVPTPHMVDD IQDEEELKRF VEAYRLLAKI 730 740 750 760 770 780 ILRLKAFDEF EFTIDEIGMD EQENEDYKSK YLAVYDQVKR ATAEKNKVSI LNDIDFEIEM 790 800 810 820 830 840 MRNDTINVNY IMNILRQIDL EDKAEQRRNQ EQIRRILDHA DDPTLRLKRD LIREFIDNVV 850 860 870 880 890 900 PSLNKDDDID QEYVNFESIK KEAEFKGFAG ERSIDEQALK TISNDYQYSG VVNPHHLKKM 910 920 IGDLPLKEKR KARKAIESFV AETTEKYGV

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW37476.1.
RefSeq	YP_185079.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HJH8.
SMR	Q5HJH8. Positions 2-783.
ModBase	Search...
Protein-protein interaction databases
STRING	93062.SACOL0180.
Protein family/group databases
REBASE	10753. SauCOLORF180P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW37476; AAW37476; SACOL0180.
GeneID	3236405.
KEGG	sac:SACOL0180.
PATRIC	19526626. VBIStaAur112458_0179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0610.
HOGENOM	HOG000003518.
KO	K01153.
OMA	NRYDVTI.
ProtClustDB	CLSK884469.
Family and domain databases
InterPro	IPR006935. Helicase/UvrB_dom. IPR014001. Helicase_ATP-bd. IPR007409. Restrct_endonuc_type1_HsdR_N. IPR004473. Restrct_endonuc_typeI_HsdR. IPR022625. TypeI_RM_Rsu_C. [Graphical view]
Pfam	PF12008. EcoR124_C. 1 hit. PF04313. HSDR_N. 1 hit. PF04851. ResIII. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00348. hsdR. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HSDR_STAAC

Accession

Primary (citable) accession number: Q5HJH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program