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Protein

L-lactate dehydrogenase 1

Gene

ldh1

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells.1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei124NAD or substrateBy similarity1
Binding sitei155SubstrateBy similarity1
Active sitei179Proton acceptorBy similarity1
Binding sitei232SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 43NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1 (EC:1.1.1.27)
Short name:
L-LDH 1
Gene namesi
Name:ldh1
Ordered Locus Names:SACOL0222
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683791 – 317L-lactate dehydrogenase 1Add BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By nitrosative stress and anaerobiosis. Expression is not detected during aerobic growth.1 Publication

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi93062.SACOL0222.

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Helixi16 – 28Combined sources13
Beta strandi32 – 37Combined sources6
Helixi41 – 54Combined sources14
Helixi55 – 57Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi63 – 66Combined sources4
Helixi69 – 72Combined sources4
Beta strandi76 – 80Combined sources5
Helixi92 – 112Combined sources21
Beta strandi117 – 121Combined sources5
Beta strandi123 – 125Combined sources3
Helixi126 – 137Combined sources12
Helixi141 – 143Combined sources3
Beta strandi144 – 146Combined sources3
Helixi150 – 164Combined sources15
Helixi168 – 170Combined sources3
Beta strandi175 – 177Combined sources3
Turni187 – 189Combined sources3
Helixi197 – 202Combined sources6
Helixi207 – 219Combined sources13
Helixi221 – 229Combined sources9
Helixi234 – 248Combined sources15
Beta strandi253 – 263Combined sources11
Helixi264 – 266Combined sources3
Beta strandi268 – 279Combined sources12
Beta strandi282 – 286Combined sources5
Helixi293 – 311Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D0OX-ray1.80A/B1-317[»]
3D4PX-ray1.80A/B1-317[»]
3H3JX-ray1.80A/B1-317[»]
ProteinModelPortaliQ5HJD7.
SMRiQ5HJD7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5HJD7.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HJD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD
60 70 80 90 100
LKHATPYSPT TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL
110 120 130 140 150
KIFKSIVGEV MASKFDGIFL VATNPVDILA YATWKFSGLP KERVIGSGTI
160 170 180 190 200
LDSARFRLLL SEAFDVAPRS VDAQIIGEHG DTELPVWSHA NIAGQPLKTL
210 220 230 240 250
LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL ARITEAIFRN
260 270 280 290 300
EDAVLTVSAL LEGEYEEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA
310
HSAKTLKDIM AEAEELK
Length:317
Mass (Da):34,583
Last modified:February 15, 2005 - v1
Checksum:iCDDE481269B7DE56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38779.1.
RefSeqiWP_001031882.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38779; AAW38779; SACOL0222.
GeneIDi28379444.
KEGGisac:SACOL0222.
PATRICi19526706. VBIStaAur112458_0219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38779.1.
RefSeqiWP_001031882.1. NC_002951.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D0OX-ray1.80A/B1-317[»]
3D4PX-ray1.80A/B1-317[»]
3H3JX-ray1.80A/B1-317[»]
ProteinModelPortaliQ5HJD7.
SMRiQ5HJD7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW38779; AAW38779; SACOL0222.
GeneIDi28379444.
KEGGisac:SACOL0222.
PATRICi19526706. VBIStaAur112458_0219.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiMDLMQTA.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Miscellaneous databases

EvolutionaryTraceiQ5HJD7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH1_STAAC
AccessioniPrimary (citable) accession number: Q5HJD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.