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Protein

L-lactate dehydrogenase 1

Gene

ldh1

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate (Potential). Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells (PubMed:18356528).UniRule annotation1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 1 (ldh1), L-lactate dehydrogenase 2 (ldh2)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38NADUniRule annotationCombined sources2 Publications1
Binding sitei43NADUniRule annotation1
Binding sitei69NADUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei92SubstrateUniRule annotationCombined sources1 Publication1
Binding sitei105NADUniRule annotation1
Binding sitei147NADUniRule annotationCombined sources1 Publication1
Active sitei179Proton acceptorUniRule annotationCombined sources2 Publications1
Binding sitei232SubstrateUniRule annotationCombined sources2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 17NADCombined sources2 Publications2
Nucleotide bindingi83 – 84NADUniRule annotationCombined sources2 Publications2
Nucleotide bindingi122 – 124NADUniRule annotationCombined sources1 Publication3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 11 PublicationUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDH 11 PublicationUniRule annotation
Gene namesi
Name:ldh1UniRule annotation
Ordered Locus Names:SACOL0222
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683791 – 317L-lactate dehydrogenase 1Add BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By nitrosative stress and anaerobiosis. Expression is not detected during aerobic growth.1 Publication

Interactioni

Subunit structurei

Homotetramer.UniRule annotation2 Publications

Protein-protein interaction databases

STRINGi93062.SACOL0222

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Helixi16 – 28Combined sources13
Beta strandi32 – 37Combined sources6
Helixi41 – 54Combined sources14
Helixi55 – 57Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi63 – 66Combined sources4
Helixi69 – 72Combined sources4
Beta strandi76 – 80Combined sources5
Helixi92 – 112Combined sources21
Beta strandi117 – 121Combined sources5
Beta strandi123 – 125Combined sources3
Helixi126 – 137Combined sources12
Helixi141 – 143Combined sources3
Beta strandi144 – 146Combined sources3
Helixi150 – 164Combined sources15
Helixi168 – 170Combined sources3
Beta strandi175 – 177Combined sources3
Turni187 – 189Combined sources3
Helixi197 – 202Combined sources6
Helixi207 – 219Combined sources13
Helixi221 – 229Combined sources9
Helixi234 – 248Combined sources15
Beta strandi253 – 263Combined sources11
Helixi264 – 266Combined sources3
Beta strandi268 – 279Combined sources12
Beta strandi282 – 286Combined sources5
Helixi293 – 311Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D0OX-ray1.80A/B1-317[»]
3D4PX-ray1.80A/B1-317[»]
3H3JX-ray1.80A/B1-317[»]
ProteinModelPortaliQ5HJD7
SMRiQ5HJD7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5HJD7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingUniRule annotation4
Regioni152 – 155Substrate bindingUniRule annotationCombined sources2 Publications4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q5HJD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD
60 70 80 90 100
LKHATPYSPT TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL
110 120 130 140 150
KIFKSIVGEV MASKFDGIFL VATNPVDILA YATWKFSGLP KERVIGSGTI
160 170 180 190 200
LDSARFRLLL SEAFDVAPRS VDAQIIGEHG DTELPVWSHA NIAGQPLKTL
210 220 230 240 250
LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL ARITEAIFRN
260 270 280 290 300
EDAVLTVSAL LEGEYEEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA
310
HSAKTLKDIM AEAEELK
Length:317
Mass (Da):34,583
Last modified:February 15, 2005 - v1
Checksum:iCDDE481269B7DE56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA Translation: AAW38779.1
RefSeqiWP_001031882.1, NC_002951.2

Genome annotation databases

EnsemblBacteriaiAAW38779; AAW38779; SACOL0222
KEGGisac:SACOL0222

Similar proteinsi

Entry informationi

Entry nameiLDH1_STAAC
AccessioniPrimary (citable) accession number: Q5HJD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: February 15, 2005
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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