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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1

Gene

ispD1

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei14 – 141Transition state stabilizerUniRule annotation
Sitei22 – 221Transition state stabilizerUniRule annotation
Sitei160 – 1601Positions MEP for the nucleophilic attackUniRule annotation
Sitei217 – 2171Positions MEP for the nucleophilic attackUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-228-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1UniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1UniRule annotation
MEP cytidylyltransferase 1UniRule annotation
Short name:
MCT 1UniRule annotation
Gene namesi
Name:ispD1UniRule annotation
Ordered Locus Names:SACOL0236
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2382382-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1PRO_0000075613Add
BLAST

Proteomic databases

PRIDEiQ5HJC5.

Interactioni

Protein-protein interaction databases

STRINGi93062.SACOL0236.

Structurei

3D structure databases

ProteinModelPortaliQ5HJC5.
SMRiQ5HJC5. Positions 1-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108QHG. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiNDRFDKI.
OrthoDBiEOG6FFS8T.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HJC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII
60 70 80 90 100
IATPAQWISH TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDHIRNVNG
110 120 130 140 150
INNDDVIVTH DAVRPFLTQR IIKENIEVAA KYGAVDTVIE AIDTIVMSKD
160 170 180 190 200
KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS YRALSSEQKE ILSDACKIIV
210 220 230
ESGHAVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD
Length:238
Mass (Da):26,575
Last modified:February 15, 2005 - v1
Checksum:iA2C4EB712DFC764E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38791.1.
RefSeqiWP_000638475.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38791; AAW38791; SACOL0236.
KEGGisac:SACOL0236.
PATRICi19526728. VBIStaAur112458_0230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38791.1.
RefSeqiWP_000638475.1. NC_002951.2.

3D structure databases

ProteinModelPortaliQ5HJC5.
SMRiQ5HJC5. Positions 1-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0236.

Proteomic databases

PRIDEiQ5HJC5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW38791; AAW38791; SACOL0236.
KEGGisac:SACOL0236.
PATRICi19526728. VBIStaAur112458_0230.

Phylogenomic databases

eggNOGiENOG4108QHG. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiNDRFDKI.
OrthoDBiEOG6FFS8T.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciSAUR93062:GCEP-228-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COL.

Entry informationi

Entry nameiISPD1_STAAC
AccessioniPrimary (citable) accession number: Q5HJC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 15, 2005
Last modified: March 16, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.