Q5HJ99 (LYTM_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycyl-glycine endopeptidase lytM
EC=3.4.24.75

Alternative name(s):
Autolysin lytM

Gene names

Name:	lytM
Ordered Locus Names:	SACOL0263

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan By similarity.
Catalytic activity	Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase M23B family.
Sequence caution	The sequence AAW38817.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Virulence
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 316	291	Glycyl-glycine endopeptidase lytM		PRO_0000026820
Sites
	Metal binding	117	1	Zinc By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	293	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HJ99 [UniParc]. Last modified June 21, 2005. Version 2. Checksum: 9974F78A19522C09	FASTA	316	34,317
	10 20 30 40 50 60 MKKLTAAAIA TMGFATFTMA HQADAAETTN TQQAHTQMST QSQDVSYGTY YTIDSNGDYH 70 80 90 100 110 120 HTPDGNWNQA MFDNKEYSYT FVDAQGHTHY FYNCYPKNAN ANGSGQTYVN PATAGDNNDY 130 140 150 160 170 180 TASQSQQHIN QYGYQSNVGP DASYYSHSNN NQAYNSHDGN GKVNYPNGTS NQNGGSASKA 190 200 210 220 230 240 TASGHAKDAS WLTSRKQLQP YGQYHGGGAH YGVDYAMPEN SPVYSLTDGT VVQAGWSNYG 250 260 270 280 290 300 GGNQVTIKEA NSNNYQWYMH NNRLTVSAGD KVKAGDQIAY SGSTGNSTAP HVHFQRMSGG 310 IGNQYAVDPT SYLQSR

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW38817.1. Different initiation.
RefSeq	YP_185158.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HJ99.
SMR	Q5HJ99. Positions 45-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HJ99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000008349; EBSTAP00000008084; EBSTAG00000008348.
GeneID	3236682.
GenomeReviews	Gene locus SACOL0263 in contig CP000046_GR.
KEGG	sac:SACOL0263.
PATRIC	19526780. VBIStaAur112458_0256.
TIGR	SACOL0263.
Phylogenomic databases
eggNOG	COG3942.
GeneTree	EBGT00050000023683.
HOGENOM	HBG693570.
OMA	IQEANSN.
ProtClustDB	CLSK884525.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL0263-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR016047. Peptidase_M23. [Graphical view]
KO	K08259.
Pfam	PF01551. Peptidase_M23. 1 hit. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYTM_STAAC

Accession

Primary (citable) accession number: Q5HJ99

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program