Q5HJ48 (LIP2_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipase 2
EC=3.1.1.3

Alternative name(s):
Glycerol ester hydrolase 2

Gene names

Name:	lip2
Synonyms:	geh
Ordered Locus Names:	SACOL0317/SACOL0390

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.
Caution	In strain COL, lip2 is interrupted in position 645 by the insertion of the L54a prophage.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Propeptide	38 – 295	258	By similarity		PRO_0000045186
	Chain	296 – 690	395	Lipase 2		PRO_0000045187
Sites
	Active site	412	1	Charge relay system By similarity
	Active site	645	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HJ48 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 8E8E3654D0E01A3B	FASTA	690	76,388
	10 20 30 40 50 60 MLRGQEERKY SIRKYSIGVV SVLAATMFVV SSHEAQASEK TSTNAAAQKE TLNQPGEQGN 70 80 90 100 110 120 AITSHQMQSG KQLDDMHKEN GKSGTVTEGK DTLQSSKHQS TQNSKTIRTQ NDNQVKQDSE 130 140 150 160 170 180 RQGSKQSHQN NATNNTERQN DQVQNTHHAE RNGSQSTTSQ SNDVDKSQPS IPAQKVIPNH 190 200 210 220 230 240 DKAAPTSTTP PSNDKTAPKS TKAQDATTDK HPNQQDTHQP AHQIIDAKQD DTVRQSEQKP 250 260 270 280 290 300 QVGDLSKHID GQNSPEKPTD KNTDNKQLIK DALQAPKTRS TTNAAADAKK VRPLKANQVQ 310 320 330 340 350 360 PLNKYPVVFV HGFLGLVGDN APALYPNYWG GNKFKVIEEL RKQGYNVHQA SVSAFGSNYD 370 380 390 400 410 420 RAVELYYYIK GGRVDYGAAH AAKYGHERYG KTYKGIMPNW EPGKKVHLVG HSMGGQTIRL 430 440 450 460 470 480 MEEFLRNGNK EEIAYHKAHG GEISPLFTGG HNNMVASITT LATPHNGSQA ADKFGNTEAV 490 500 510 520 530 540 RKIMFALNRF MGNKYSNIDL GLTQWGFKQL PNESYIDYIK RVSKSKIWTS DDNAAYDLTL 550 560 570 580 590 600 DGSAKLNNMT SMNPNITYTT YTGVSSHTGP LGYENPDLGT FFLMATTSRI IGHDAREEWR 610 620 630 640 650 660 KNDGVVPVIS SLHPSNQPFV NVTNDEPATR RGIWQVKPII QGWDHVDFIG VDFLDFKRKG 670 680 690 AELANFYTGI INDLLRVEAT ESKGTQLKAS

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW37524.1. Sequence problems. CP000046 Genomic DNA. Translation: AAW38859.1. Sequence problems.
RefSeq	YP_185209.1. NC_002951.2. YP_185282.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HJ48.
SMR	Q5HJ48. Positions 295-682.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HJ48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000009071; EBSTAP00000008806; EBSTAG00000009070.
GeneID	3236633. 3236931.
PATRIC	19526888. VBIStaAur112458_0310.
TIGR	SACOL0317. SACOL0390.
Phylogenomic databases
eggNOG	COG1075.
GeneTree	EBGT00050000023709.
OMA	ETINRIG.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL0317-MONOMER. SAUR93062:SACOL0390-MONOMER.
Family and domain databases
InterPro	IPR005877. Gpos_YSIRK. [Graphical view]
Pfam	PF04650. YSIRK_signal. 1 hit. [Graphical view]
TIGRFAMs	TIGR01168. YSIRK_signal. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIP2_STAAC

Accession

Primary (citable) accession number: Q5HJ48
Secondary accession number(s): Q5HIX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program