Reviewed, UniProtKB/Swiss-Prot Q5HIU0 (THLA_STAAC)

Names and origin

Protein names

Recommended name:
    Probable acetyl-CoA acyltransferase
    EC=2.3.1.9
Alternative name(s):
    Acetoacetyl-CoA thiolase

Gene names

Ordered Locus Names:

SACOL0426

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Probable acetyl-CoA acyltransferase		PRO_0000270503
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	349	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HIU0-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 3C1C71861BD9D381	FASTA	393	41,676
	10 20 30 40 50 60 MTRVVLAAAY RTPIGVFGGA FKDVPAYDLG ATLIEHIIKE TGLNPSEIDE VIIGNVLQAG 70 80 90 100 110 120 QGQNPARIAA MKGGLPETVP AFTVNKVCGS GLKSIQLAYQ SIVTGENDIV LAGGMENMSQ 130 140 150 160 170 180 SPMLVNNSRF GFKMGHQSMV DSMVYDGLTD VFNQYHMGIT AENLAEQYGI SREEQDTFAV 190 200 210 220 230 240 NSQQKAVRAQ QNGEFDSEIV PVSIPQRKGE PIVVTKDEGV RENVSVEKLS RLRPAFKKDG 250 260 270 280 290 300 TVTAGNASGI NDGAAMMLVM SEDKAKELNI EPLAVLDGFG SHGVDPSIMG IAPVGAVEKA 310 320 330 340 350 360 LKRSKKELSD IDVFELNEAF AAQSLAVDRE LKLPPEKVNV KGGAIALGHP IGASGARVLV 370 380 390 TLLHQLNDEV ETGLTSLCIG GGQAIAAVVS KYK

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW38894.1.
RefSeq	YP_185317.1.
3D structure databases
HSSP	HSSP built from PDB template 1WL4 based on UniProtKB Q9BWD1.
SMR	Q5HIU0. Positions 4-390.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HIU0.
Genome annotation databases
GeneID	3236515.
GenomeReviews	Gene locus SACOL0426 in contig CP000046_GR.
KEGG	sac:SACOL0426.
TIGR	SACOL0426.
Phylogenomic databases
eggNOG	COG0183.
HOGENOM	HBG370930.
OMA	DPATMGM.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL0426-MONOMER.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 1 hit.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THLA_STAAC

Accession

Primary (citable) accession number: Q5HIU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)