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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100CalciumPROSITE-ProRule annotation1
Active sitei114By similarity1
Metal bindingi119CalciumPROSITE-ProRule annotation1
Metal bindingi120Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Active sitei122By similarity1
Active sitei166By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Gene namesi
Name:nuc
Ordered Locus Names:SACOL0860
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000004522724 – 60By similarityAdd BLAST37
ChainiPRO_000004522861 – 228ThermonucleaseAdd BLAST168

Keywords - PTMi

Zymogen

Interactioni

Protein-protein interaction databases

STRINGi93062.SACOL0860.

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi88 – 96Combined sources9
Beta strandi98 – 106Combined sources9
Beta strandi109 – 115Combined sources7
Helixi134 – 147Combined sources14
Beta strandi151 – 154Combined sources4
Beta strandi167 – 173Combined sources7
Helixi178 – 184Combined sources7
Beta strandi187 – 190Combined sources4
Helixi201 – 213Combined sources13
Helixi217 – 219Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WRDX-ray1.65A80-228[»]
5DAUX-ray1.50A80-228[»]
ProteinModelPortaliQ5HHM4.
SMRiQ5HHM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107Y9E. Bacteria.
COG1525. LUCA.
HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5HHM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYLLSAGI CMAIVSILLI GMAISNVSKG QYAKRFFYFA TSCLVLTLVV
60 70 80 90 100
VSSLSSSANA SQTDNGVNRS GSEDPTVYSA TSTKKLHKEP ATLIKAIDGD
110 120 130 140 150
TVKLMYKGQP MTFRLLLVDT PETKHPKKGV EKYGPEASAF TKKMVENAKK
160 170 180 190 200
IEVEFDKGQR TDKYGRGLAY IYADGKMVNE ALVRQGLAKV AYVYKPNNTH
210 220
EQLLRKSEAQ AKKEKLNIWS EDNADSGQ
Length:228
Mass (Da):25,120
Last modified:February 15, 2005 - v1
Checksum:i1505918EA02F1273
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW36415.1.
RefSeqiWP_001548082.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW36415; AAW36415; SACOL0860.
GeneIDi28381643.
KEGGisac:SACOL0860.
PATRICi19527968. VBIStaAur112458_0838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW36415.1.
RefSeqiWP_001548082.1. NC_002951.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WRDX-ray1.65A80-228[»]
5DAUX-ray1.50A80-228[»]
ProteinModelPortaliQ5HHM4.
SMRiQ5HHM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW36415; AAW36415; SACOL0860.
GeneIDi28381643.
KEGGisac:SACOL0860.
PATRICi19527968. VBIStaAur112458_0838.

Phylogenomic databases

eggNOGiENOG4107Y9E. Bacteria.
COG1525. LUCA.
HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAC
AccessioniPrimary (citable) accession number: Q5HHM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.