UniProtKB - Q5HH14 (PUR1_STAAC)
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Protein
Amidophosphoribosyltransferase
Gene
purF
Organism
Staphylococcus aureus (strain COL)
Status
Functioni
Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation
Catalytic activityi
5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Amidophosphoribosyltransferase (purF)
- Phosphoribosylamine--glycine ligase (purD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 11 | NucleophileUniRule annotation | 1 | |
Metal bindingi | 294 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 356 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 357 | MagnesiumUniRule annotation | 1 |
GO - Molecular functioni
- amidophosphoribosyltransferase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: UniProtKB-KW
- nucleoside metabolic process Source: InterPro
- purine nucleobase biosynthetic process Source: InterPro
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00074; UER00124. |
Names & Taxonomyi
Protein namesi | Recommended name: AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)Short name: ATaseUniRule annotation Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation Short name: GPATaseUniRule annotation |
Gene namesi | Name:purFUniRule annotation Ordered Locus Names:SACOL1079 |
Organismi | Staphylococcus aureus (strain COL) |
Taxonomic identifieri | 93062 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
PropeptideiPRO_0000045298 | 1 – 10 | By similarity | 10 | |
ChainiPRO_0000045299 | 11 – 494 | AmidophosphoribosyltransferaseAdd BLAST | 484 |
Structurei
3D structure databases
ProteinModelPortali | Q5HH14. |
SMRi | Q5HH14. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 231 | Glutamine amidotransferase type-2UniRule annotationAdd BLAST | 221 |
Sequence similaritiesi
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | ENOG4105CBA. Bacteria. COG0034. LUCA. |
HOGENOMi | HOG000033688. |
KOi | K00764. |
OMAi | FRPLCLG. |
Family and domain databases
CDDi | cd00715. GPATase_N. 1 hit. cd06223. PRTases_typeI. 1 hit. |
Gene3Di | 3.60.20.10. 3 hits. |
HAMAPi | MF_01931. PurF. 1 hit. |
InterProi | View protein in InterPro IPR017932. GATase_2_dom. IPR029055. Ntn_hydrolases_N. IPR000836. PRibTrfase_dom. IPR029057. PRTase-like. IPR005854. PurF. IPR035584. PurF_N. |
Pfami | View protein in Pfam PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit. |
PIRSFi | PIRSF000485. Amd_phspho_trans. 1 hit. |
SUPFAMi | SSF53271. SSF53271. 1 hit. SSF56235. SSF56235. 1 hit. |
TIGRFAMsi | TIGR01134. purF. 1 hit. |
PROSITEi | View protein in PROSITE PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q5HH14-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSDQNE
60 70 80 90 100
LKGERGLGLL TEAIKDDQME RLKGYQHAIG HVRYATSGNK GIENIQPFLY
110 120 130 140 150
HFYDMSVGIC HNGNLINAKS LRQNLEKQGA IFHSSSDTEV IMHLIRRSKA
160 170 180 190 200
PTFEEALKES LRKVKGGFTF AILTKDALYG AVDPNAIRPL VVGKMKDGTY
210 220 230 240 250
ILASETCAID VLGAEFVQDI HAGEYVVIND KGITVKSYTH HTTTAISAME
260 270 280 290 300
YIYFARPDST IAGKNVHAVR KASGKKLAQE SPVNADMVIG VPNSSLSAAS
310 320 330 340 350
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK
360 370 380 390 400
NIILVDDSIV RGTTIRRIVK MLKDSGANKV HVRIASPEFM FPSFYGIDVS
410 420 430 440 450
TTAELISASK SPEEIKDYIG ADSLAYLSVD GLIESIGLDY DAPYSGLCVE
460 470 480 490
SFTGDYPAGL YDYEANYKAH LSHRQKQYIS KNKHFFDSEG NLNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP000046 Genomic DNA. Translation: AAW37959.1. |
RefSeqi | WP_000483713.1. NC_002951.2. |
Genome annotation databases
EnsemblBacteriai | AAW37959; AAW37959; SACOL1079. |
KEGGi | sac:SACOL1079. |
Similar proteinsi
Entry informationi
Entry namei | PUR1_STAAC | |
Accessioni | Q5HH14Primary (citable) accession number: Q5HH14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | February 15, 2005 | |
Last modified: | March 28, 2018 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |