purF

Functionⁱ

Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.UniRule annotation

Catalytic activityⁱ

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H₂O.UniRule annotation

Cofactorⁱ

Mg²⁺UniRule annotationNote: Binds 1 Mg²⁺ ion per subunit.UniRule annotation

Pathwayⁱ: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

Amidophosphoribosyltransferase (purF)
Phosphoribosylamine--glycine ligase (purD)

This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	11	NucleophileUniRule annotation	1
Metal bindingⁱ	294	MagnesiumUniRule annotation	1
Metal bindingⁱ	356	MagnesiumUniRule annotation	1
Metal bindingⁱ	357	MagnesiumUniRule annotation	1

GO - Molecular functionⁱ

amidophosphoribosyltransferase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
glutamine metabolic process Source: UniProtKB-KW
nucleoside metabolic process Source: InterPro
purine nucleobase biosynthetic process Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Purine biosynthesis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00074; UER00124.

UniPathwayⁱ

UPA00074; UER00124.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation) Short name: ATaseUniRule annotation Alternative name(s): Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation Short name: GPATaseUniRule annotation
Gene namesⁱ	Name:purFUniRule annotation Ordered Locus Names:SACOL1079
Organismⁱ	Staphylococcus aureus (strain COL)
Taxonomic identifierⁱ	93062 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus
Proteomesⁱ	UP000000530 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
PropeptideⁱPRO_0000045298	1 – 10	By similarity		10
ChainⁱPRO_0000045299	11 – 494	AmidophosphoribosyltransferaseAdd BLAST		484

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	93062.SACOL1079.

STRINGⁱ

93062.SACOL1079.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q5HH14.
SMRⁱ	Q5HH14.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	11 – 231	Glutamine amidotransferase type-2UniRule annotationAdd BLAST		221

Sequence similaritiesⁱ

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Keywords - Domainⁱ

Glutamine amidotransferase

Phylogenomic databases

eggNOGⁱ	ENOG4105CBA. Bacteria. COG0034. LUCA.
HOGENOMⁱ	HOG000033688.
KEGG Orthology (KO) More...KOⁱ	K00764.
OMAⁱ	FRPLCLG.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00715. GPATase_N. 1 hit. cd06223. PRTases_typeI. 1 hit.
Gene3Dⁱ	3.60.20.10. 3 hits.
HAMAPⁱ	MF_01931. PurF. 1 hit.
InterProⁱ	View protein in InterPro IPR017932. GATase_2_dom. IPR029055. Ntn_hydrolases_N. IPR000836. PRibTrfase_dom. IPR029057. PRTase-like. IPR005854. PurF. IPR035584. PurF_N.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit.
PIRSFⁱ	PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMⁱ	SSF53271. SSF53271. 1 hit. SSF56235. SSF56235. 1 hit.
TIGRFAMsⁱ	TIGR01134. purF. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q5HH14-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSDQNE 
        60         70         80         90        100
LKGERGLGLL TEAIKDDQME RLKGYQHAIG HVRYATSGNK GIENIQPFLY 
       110        120        130        140        150
HFYDMSVGIC HNGNLINAKS LRQNLEKQGA IFHSSSDTEV IMHLIRRSKA 
       160        170        180        190        200
PTFEEALKES LRKVKGGFTF AILTKDALYG AVDPNAIRPL VVGKMKDGTY 
       210        220        230        240        250
ILASETCAID VLGAEFVQDI HAGEYVVIND KGITVKSYTH HTTTAISAME 
       260        270        280        290        300
YIYFARPDST IAGKNVHAVR KASGKKLAQE SPVNADMVIG VPNSSLSAAS 
       310        320        330        340        350
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK 
       360        370        380        390        400
NIILVDDSIV RGTTIRRIVK MLKDSGANKV HVRIASPEFM FPSFYGIDVS 
       410        420        430        440        450
TTAELISASK SPEEIKDYIG ADSLAYLSVD GLIESIGLDY DAPYSGLCVE 
       460        470        480        490 
SFTGDYPAGL YDYEANYKAH LSHRQKQYIS KNKHFFDSEG NLNV

Length:494

Mass (Da):54,397

Last modified:February 15, 2005 - v1

Checksum:ⁱFED58A366B36AD8A

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000046 Genomic DNA. Translation: AAW37959.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000483713.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaⁱ	AAW37959; AAW37959; SACOL1079.
KEGGⁱ	sac:SACOL1079.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q5HH14	Q6GAE3 Q8NX91 X5DX64 A0A0D1IYE5 A0A0E1AFE6 A0A0H3K814 Q2FZI9 A0A0H2XFY4 A0A1E8WRJ8 UPI000BA54A5D +1	Staphylococcus aureus (strain MSSA476) Staphylococcus aureus (strain MW2) Staphylococcus aureus Staphylococcus aureus subsp. aureus Staphylococcus aureus subsp. aureus Z172 Staphylococcus aureus (strain Newman) Staphylococcus aureus (strain NCTC 8325) Staphylococcus aureus (strain USA300) Staphylococcus sp. HMSC055H04	494	UniRef100_Q5HH14	Cluster: Amidophosphoribosyltransferase	12

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q5HH14	P65831 P99164 A0A299M4K6 UPI000BA799BB UPI000BA50FB1 UPI00044834BD T1Y7R4 UPI00040435FC UPI000BC875E3 A0A0U1MJF5 +239	Staphylococcus aureus (strain Mu50 / ATCC 700699) Staphylococcus aureus (strain N315) Staphylococcus aureus Staphylococcus aureus subsp. aureus CN1 Staphylococcus aureus subsp. aureus 71193 Staphylococcus simiae CCM 7213 Staphylococcus simiae Staphylococcus Staphylococcus capitis Staphylococcus sp. HMSC074F11 And more	494	UniRef90_P65831	Cluster: Amidophosphoribosyltransferase	250

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q5HH14	P65831 P99164 A0A299M4K6 UPI000BA799BB UPI000BA50FB1 UPI00044834BD A0A0U1MJF5 UPI00059F5A8A UPI0002477980 UPI000BA79AE5 +1181	Staphylococcus aureus (strain Mu50 / ATCC 700699) Staphylococcus aureus (strain N315) Staphylococcus aureus Staphylococcus aureus subsp. aureus CN1 Staphylococcus aureus subsp. aureus 71193 Staphylococcus epidermidis Staphylococcus argenteus Staphylococcus Staphylococcus warneri L37603 Staphylococcus capitis And more	494	UniRef50_P65831	Cluster: Amidophosphoribosyltransferase	1,192

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP000046 Genomic DNA. Translation: AAW37959.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_000483713.1. NC_002951.2.

3D structure databases

ProteinModelPortalⁱ	Q5HH14.
SMRⁱ	Q5HH14.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	93062.SACOL1079.

STRINGⁱ

93062.SACOL1079.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAW37959; AAW37959; SACOL1079.
KEGGⁱ	sac:SACOL1079.

Phylogenomic databases

eggNOGⁱ	ENOG4105CBA. Bacteria. COG0034. LUCA.
HOGENOMⁱ	HOG000033688.
KEGG Orthology (KO) More...KOⁱ	K00764.
OMAⁱ	FRPLCLG.

Enzyme and pathway databases

UniPathwayⁱ	UPA00074; UER00124.

UniPathwayⁱ

UPA00074; UER00124.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00715. GPATase_N. 1 hit. cd06223. PRTases_typeI. 1 hit.
Gene3Dⁱ	3.60.20.10. 3 hits.
HAMAPⁱ	MF_01931. PurF. 1 hit.
InterProⁱ	View protein in InterPro IPR017932. GATase_2_dom. IPR029055. Ntn_hydrolases_N. IPR000836. PRibTrfase_dom. IPR029057. PRTase-like. IPR005854. PurF. IPR035584. PurF_N.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit.
PIRSFⁱ	PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMⁱ	SSF53271. SSF53271. 1 hit. SSF56235. SSF56235. 1 hit.
TIGRFAMsⁱ	TIGR01134. purF. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PUR1_STAAC
Accessionⁱ	Q5HH14Primary (citable) accession number: Q5HH14
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	February 15, 2005
	Last modified:	March 28, 2018
	This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q5HH14 (PUR1_STAAC)

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Amidophosphoribosyltransferase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: IMP biosynthesis via de novo pathway

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: IMP biosynthesis via de novo pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ