Reviewed, UniProtKB/Swiss-Prot Q5HH10 (PUR2_STAAC)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	SACOL1083

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151478
Regions
	Domain	108 – 311	204	ATP-grasp
	Nucleotide binding	134 – 191	58	ATP By similarity
Sites
	Metal binding	281	1	Magnesium or manganese By similarity
	Metal binding	283	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HH10-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: E4C137A5A1187C27	FASTA	415	45,859
	10 20 30 40 50 60 MNVLVIGAGG REHALAYKLN QSNLVKQVFV IPGNEAMTPI AEVHTEISEP DHQAILDFAK 70 80 90 100 110 120 RQNVDWVVIG PEQPLIDGLA DILRANGFKV FGPNKQAAQI EGSKLFAKKI MEKYNIPTAD 130 140 150 160 170 180 YKEVERKKDA LTYIENCELP VVVKKDGLAA GKGVIIADTI EAARSAIEIM YGDEEEGTVV 190 200 210 220 230 240 FETFLEGEEF SLMTFVNGDL AVPFDCIAQD HKRAFDHDEG PNTGGMGAYC PVPHISDDVL 250 260 270 280 290 300 KLTNETIAQP IAKAMLNEGY QFFGVLYIGA ILTKDGPKVI EFNARFGDPE AQVLLSRMES 310 320 330 340 350 360 DLMQHIIDLD EGKRTEFKWK NESIVGVMLA SKGYPDAYEK GHKVSGFDLN ENYFVSGLKK 370 380 390 400 410 QGDTFVTSGG RVILAIGKGD NVQDAQRDAY KKVSQIQSDH LFYRHDIANK ALQLK

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW37963.1.
RefSeq	YP_185947.1.
3D structure databases
SMR	Q5HH10. Positions 1-413.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HH10.
Genome annotation databases
GeneID	3236107.
GenomeReviews	Gene locus SACOL1083 in contig CP000046_GR.
KEGG	sac:SACOL1083.
TIGR	SACOL1083.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL1083-MONOMER.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_STAAC

Accession

Primary (citable) accession number: Q5HH10

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)