Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q5HGI2 (TOP1_STAAC)

Last modified November 3, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase
Gene names
Name: topA
Ordered Locus Names: SACOL1267
OrganismStaphylococcus aureus (strain COL) [Complete proteome] [HAMAP]
Taxonomic identifier93062 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesStaphylococcus

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Miscellaneous

When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand By similarity.

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689DNA topoisomerase 1
PRO_0000285936

Regions

Zinc finger577 – 60327C4-type 1
Zinc finger617 – 64529C4-type 2
Zinc finger658 – 68124C4-type 3

Sites

Active site2981For DNA cleavage activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HGI2-1 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 1F28D3BC10BDADE1

FASTA68979,113
        10         20         30         40         50         60 
MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDTEDNY EPKYITIRGK 

        70         80         90        100        110        120 
GPVVKELKKH AKKAKNVFLA SDPDREGEAI AWHLSKILEL EDSKENRVVF NEITKDAVKE 

       130        140        150        160        170        180 
SFKNPREIEM NLVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 

       190        200        210        220        230        240 
EIRNFKPEEY WTIEGEFRYK KSKFNAKFLH YKNKPFKLKT KKDVEKITAA LDGDQFEITN 

       250        260        270        280        290        300 
VTKKEKTRNP ANPFTTSTLQ QEAARKLNFK ARKTMMVAQQ LYEGIDLKKQ GTIGLITYMR 

       310        320        330        340        350        360 
TDSTRISDTA KVEAKQYITD KYGESYTSKR KASGKQGDQD AHEAIRPSST MRTPDDMKSF 

       370        380        390        400        410        420 
LTKDQYRLYK LIWERFVASQ MAPAILDTVS LDITQGDIKF RANGQTIKFK GFMTLYVETK 

       430        440        450        460        470        480 
DDSDSEKENK LPKLEQGDKV TATQIEPAQH YTQPPPRYTE ARLVKTLEEL KIGRPSTYAP 

       490        500        510        520        530        540 
TIDTIQKRNY VKLESKRFVP TELGEIVHEQ VKEYFPEIID VEFTVNMETL LDKIAEGDIT 

       550        560        570        580        590        600 
WRKVIDGFFS SFKQDVERAE EEMEKIEIKD EPAGEDCEIC GSPMVIKMGR YGKFMACSNF 

       610        620        630        640        650        660 
PDCRNTKAIV KSIGVKCPKC NDGDVVERKS KKNRVFYGCS KYPECDFISW DKPIGRDCPK 

       670        680 
CNQYLVENKK GKTTQVICSN CDYKEAAQK 

« Hide

References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

CP000046 Genomic DNA. Translation: AAW38099.1.
RefSeqYP_186125.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ5HGI2.

Genome annotation databases

GeneID3238359.
GenomeReviewsGene locus SACOL1267 in contig CP000046_GR.
KEGGsac:SACOL1267.
TIGRSACOL1267.

Phylogenomic databases

HOGENOMQ5HGI2.
OMARKFYGCS.

Enzyme and pathway databases

BioCycSAUR93062:SACOL1267-MON.

Family and domain databases

InterProIPR003601. Topo_IA_2.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR000380. Topo_IA_core.
IPR003602. Topo_IA_DNA_bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac.
IPR006154. Toprim_dom_subgr.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 3 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_STAAC
AccessionPrimary (citable) accession number: Q5HGI2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 15, 2005
Last modified: November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents