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Q5HGI2 (TOP1_STAAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase 1
EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:SACOL1267
OrganismStaphylococcus aureus (strain COL) [Complete proteome] [HAMAP]
Taxonomic identifier93062 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Staphylococcus ›

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689DNA topoisomerase 1
PRO_0000285936

Regions

Domain3 – 113111Toprim
Zinc finger577 – 60327C4-type 1
Zinc finger617 – 64529C4-type 2
Zinc finger658 – 68124C4-type 3
Region163 – 1686Interaction with DNA By similarity

Sites

Active site2981O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding821Magnesium 1; catalytic By similarity
Metal binding821Magnesium 2 By similarity
Metal binding841Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1391Interaction with DNA By similarity
Site1401Interaction with DNA By similarity
Site1431Interaction with DNA By similarity
Site1481Interaction with DNA By similarity
Site1551Interaction with DNA By similarity
Site3001Interaction with DNA By similarity
Site4881Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HGI2 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: 1F28D3BC10BDADE1

FASTA68979,113
        10         20         30         40         50         60 
MADNLVIVES PAKAKTIEKY LGKKYKVIAS MGHVRDLPRS QMGVDTEDNY EPKYITIRGK 

        70         80         90        100        110        120 
GPVVKELKKH AKKAKNVFLA SDPDREGEAI AWHLSKILEL EDSKENRVVF NEITKDAVKE 

       130        140        150        160        170        180 
SFKNPREIEM NLVDAQQARR ILDRLVGYNI SPVLWKKVKK GLSAGRVQSV ALRLVIDREN 

       190        200        210        220        230        240 
EIRNFKPEEY WTIEGEFRYK KSKFNAKFLH YKNKPFKLKT KKDVEKITAA LDGDQFEITN 

       250        260        270        280        290        300 
VTKKEKTRNP ANPFTTSTLQ QEAARKLNFK ARKTMMVAQQ LYEGIDLKKQ GTIGLITYMR 

       310        320        330        340        350        360 
TDSTRISDTA KVEAKQYITD KYGESYTSKR KASGKQGDQD AHEAIRPSST MRTPDDMKSF 

       370        380        390        400        410        420 
LTKDQYRLYK LIWERFVASQ MAPAILDTVS LDITQGDIKF RANGQTIKFK GFMTLYVETK 

       430        440        450        460        470        480 
DDSDSEKENK LPKLEQGDKV TATQIEPAQH YTQPPPRYTE ARLVKTLEEL KIGRPSTYAP 

       490        500        510        520        530        540 
TIDTIQKRNY VKLESKRFVP TELGEIVHEQ VKEYFPEIID VEFTVNMETL LDKIAEGDIT 

       550        560        570        580        590        600 
WRKVIDGFFS SFKQDVERAE EEMEKIEIKD EPAGEDCEIC GSPMVIKMGR YGKFMACSNF 

       610        620        630        640        650        660 
PDCRNTKAIV KSIGVKCPKC NDGDVVERKS KKNRVFYGCS KYPECDFISW DKPIGRDCPK 

       670        680 
CNQYLVENKK GKTTQVICSN CDYKEAAQK

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References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: COL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000046 Genomic DNA. Translation: AAW38099.1.
RefSeqYP_186125.1. NC_002951.2.

3D structure databases

HSSPHSSP built from PDB template 1ECL based on UniProtKB P06612.
ProteinModelPortalQ5HGI2.
SMRQ5HGI2. Positions 5-609.
ModBaseSearch...

Protein-protein interaction databases

STRING93062.SACOL1267.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW38099; AAW38099; SACOL1267.
GeneID3238359.
KEGGsac:SACOL1267.
PATRIC19528773. VBIStaAur112458_1239.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAEFWDIHA.
ProtClustDBPRK05582.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_STAAC
AccessionPrimary (citable) accession number: Q5HGI2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: February 15, 2005
Last modified: May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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