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Protein

Glycerol kinase

Gene

glpK

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzsyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate (By similarity).By similarity

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12SubstrateUniRule annotation1
Binding sitei16ATPUniRule annotation1
Binding sitei134Substrate1
Binding sitei266ATP1
Binding sitei309ATP; via carbonyl oxygen1
Binding sitei313ATP; via amide nitrogen1
Binding sitei328ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14ATP3
Nucleotide bindingi410 – 414ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:SACOL1320
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000594891 – 498Glycerol kinaseAdd BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230Phosphohistidine; by HPrUniRule annotation1

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).2 Publications

Protein-protein interaction databases

STRINGi93062.SACOL1320.

Structurei

Secondary structure

1498
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi12 – 20Combined sources9
Beta strandi26 – 33Combined sources8
Helixi48 – 64Combined sources17
Turni65 – 67Combined sources3
Helixi70 – 72Combined sources3
Beta strandi73 – 80Combined sources8
Beta strandi85 – 89Combined sources5
Turni90 – 92Combined sources3
Beta strandi95 – 97Combined sources3
Helixi108 – 116Combined sources9
Helixi120 – 127Combined sources8
Helixi136 – 146Combined sources11
Helixi150 – 155Combined sources6
Beta strandi159 – 163Combined sources5
Helixi164 – 172Combined sources9
Turni173 – 175Combined sources3
Beta strandi179 – 181Combined sources3
Helixi182 – 185Combined sources4
Beta strandi188 – 192Combined sources5
Turni193 – 196Combined sources4
Helixi200 – 206Combined sources7
Helixi210 – 212Combined sources3
Beta strandi215 – 217Combined sources3
Beta strandi219 – 225Combined sources7
Helixi228 – 230Combined sources3
Turni231 – 233Combined sources3
Beta strandi237 – 243Combined sources7
Helixi244 – 251Combined sources8
Beta strandi260 – 273Combined sources14
Beta strandi282 – 284Combined sources3
Beta strandi286 – 293Combined sources8
Beta strandi296 – 306Combined sources11
Helixi310 – 317Combined sources8
Helixi325 – 327Combined sources3
Helixi328 – 332Combined sources5
Beta strandi342 – 344Combined sources3
Turni352 – 354Combined sources3
Beta strandi361 – 366Combined sources6
Helixi372 – 397Combined sources26
Beta strandi403 – 409Combined sources7
Helixi410 – 413Combined sources4
Helixi415 – 425Combined sources11
Beta strandi427 – 433Combined sources7
Helixi437 – 449Combined sources13
Beta strandi452 – 454Combined sources3
Helixi457 – 462Combined sources6
Beta strandi465 – 469Combined sources5
Helixi475 – 494Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G25X-ray1.90A/B/C/D1-498[»]
3GE1X-ray2.70A/B/C/D1-498[»]
ProteinModelPortaliQ5HGD2.
SMRiQ5HGD2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5HGD2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 83Substrate binding2
Regioni244 – 245Substrate binding2

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HGD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYILSIDQ GTTSSRAILF NQKGEIAGVA QREFKQYFPQ SGWVEHDANE
60 70 80 90 100
IWTSVLAVMT EVINENDVRA DQIAGIGITN QRETTVVWDK HTGRPIYHAI
110 120 130 140 150
VWQSRQTQSI CSELKQQGYE QTFRDKTGLL LDPYFAGTKV KWILDNVEGA
160 170 180 190 200
REKAENGDLL FGTIDTWLVW KLSGKAAHIT DYSNASRTLM FNIHDLEWDD
210 220 230 240 250
ELLELLTVPK NMLPEVKASS EVYGKTIDYH FYGQEVPIAG VAGDQQAALF
260 270 280 290 300
GQACFERGDV KNTYGTGGFM LMNTGDKAVK SESGLLTTIA YGIDGKVNYA
310 320 330 340 350
LEGSIFVSGS AIQWLRDGLR MINSAPQSES YATRVDSTEG VYVVPAFVGL
360 370 380 390 400
GTPYWDSEAR GAIFGLTRGT EKEHFIRATL ESLCYQTRDV MEAMSKDSGI
410 420 430 440 450
DVQSLRVDGG AVKNNFIMQF QADIVNTSVE RPEIQETTAL GAAFLAGLAV
460 470 480 490
GFWESKDDIA KNWKLEEKFD PKMDEGEREK LYRGWKKAVE ATQVFKTE
Length:498
Mass (Da):55,626
Last modified:February 15, 2005 - v1
Checksum:i6B11099ED0AFB561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38149.1.
RefSeqiWP_000417369.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38149; AAW38149; SACOL1320.
GeneIDi28381140.
KEGGisac:SACOL1320.
PATRICi19528871. VBIStaAur112458_1288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38149.1.
RefSeqiWP_000417369.1. NC_002951.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3G25X-ray1.90A/B/C/D1-498[»]
3GE1X-ray2.70A/B/C/D1-498[»]
ProteinModelPortaliQ5HGD2.
SMRiQ5HGD2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL1320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW38149; AAW38149; SACOL1320.
GeneIDi28381140.
KEGGisac:SACOL1320.
PATRICi19528871. VBIStaAur112458_1288.

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.

Miscellaneous databases

EvolutionaryTraceiQ5HGD2.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_STAAC
AccessioniPrimary (citable) accession number: Q5HGD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.