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Protein

Glycerol kinase

Gene

glpK

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Key enzsyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate (By similarity).By similarity

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Activated by phosphorylation and inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121SubstrateUniRule annotation
Binding sitei16 – 161ATPUniRule annotation
Binding sitei134 – 1341Substrate
Binding sitei266 – 2661ATP
Binding sitei309 – 3091ATP; via carbonyl oxygen
Binding sitei313 – 3131ATP; via amide nitrogen
Binding sitei328 – 3281ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 143ATP
Nucleotide bindingi410 – 4145ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-1298-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:SACOL1320
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Glycerol kinasePRO_0000059489Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301Phosphohistidine; by HPrUniRule annotation

Post-translational modificationi

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), including enzyme I, and histidine-containing protein (HPr) are required for the phosphorylation, which leads to the activation of the enzyme.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer and homodimer (in equilibrium).2 Publications

Protein-protein interaction databases

STRINGi93062.SACOL1320.

Structurei

Secondary structure

1
498
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 107Combined sources
Beta strandi12 – 209Combined sources
Beta strandi26 – 338Combined sources
Helixi48 – 6417Combined sources
Turni65 – 673Combined sources
Helixi70 – 723Combined sources
Beta strandi73 – 808Combined sources
Beta strandi85 – 895Combined sources
Turni90 – 923Combined sources
Beta strandi95 – 973Combined sources
Helixi108 – 1169Combined sources
Helixi120 – 1278Combined sources
Helixi136 – 14611Combined sources
Helixi150 – 1556Combined sources
Beta strandi159 – 1635Combined sources
Helixi164 – 1729Combined sources
Turni173 – 1753Combined sources
Beta strandi179 – 1813Combined sources
Helixi182 – 1854Combined sources
Beta strandi188 – 1925Combined sources
Turni193 – 1964Combined sources
Helixi200 – 2067Combined sources
Helixi210 – 2123Combined sources
Beta strandi215 – 2173Combined sources
Beta strandi219 – 2257Combined sources
Helixi228 – 2303Combined sources
Turni231 – 2333Combined sources
Beta strandi237 – 2437Combined sources
Helixi244 – 2518Combined sources
Beta strandi260 – 27314Combined sources
Beta strandi282 – 2843Combined sources
Beta strandi286 – 2938Combined sources
Beta strandi296 – 30611Combined sources
Helixi310 – 3178Combined sources
Helixi325 – 3273Combined sources
Helixi328 – 3325Combined sources
Beta strandi342 – 3443Combined sources
Turni352 – 3543Combined sources
Beta strandi361 – 3666Combined sources
Helixi372 – 39726Combined sources
Beta strandi403 – 4097Combined sources
Helixi410 – 4134Combined sources
Helixi415 – 42511Combined sources
Beta strandi427 – 4337Combined sources
Helixi437 – 44913Combined sources
Beta strandi452 – 4543Combined sources
Helixi457 – 4626Combined sources
Beta strandi465 – 4695Combined sources
Helixi475 – 49420Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G25X-ray1.90A/B/C/D1-498[»]
3GE1X-ray2.70A/B/C/D1-498[»]
ProteinModelPortaliQ5HGD2.
SMRiQ5HGD2. Positions 1-498.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5HGD2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 832Substrate binding
Regioni244 – 2452Substrate binding

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HGD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYILSIDQ GTTSSRAILF NQKGEIAGVA QREFKQYFPQ SGWVEHDANE
60 70 80 90 100
IWTSVLAVMT EVINENDVRA DQIAGIGITN QRETTVVWDK HTGRPIYHAI
110 120 130 140 150
VWQSRQTQSI CSELKQQGYE QTFRDKTGLL LDPYFAGTKV KWILDNVEGA
160 170 180 190 200
REKAENGDLL FGTIDTWLVW KLSGKAAHIT DYSNASRTLM FNIHDLEWDD
210 220 230 240 250
ELLELLTVPK NMLPEVKASS EVYGKTIDYH FYGQEVPIAG VAGDQQAALF
260 270 280 290 300
GQACFERGDV KNTYGTGGFM LMNTGDKAVK SESGLLTTIA YGIDGKVNYA
310 320 330 340 350
LEGSIFVSGS AIQWLRDGLR MINSAPQSES YATRVDSTEG VYVVPAFVGL
360 370 380 390 400
GTPYWDSEAR GAIFGLTRGT EKEHFIRATL ESLCYQTRDV MEAMSKDSGI
410 420 430 440 450
DVQSLRVDGG AVKNNFIMQF QADIVNTSVE RPEIQETTAL GAAFLAGLAV
460 470 480 490
GFWESKDDIA KNWKLEEKFD PKMDEGEREK LYRGWKKAVE ATQVFKTE
Length:498
Mass (Da):55,626
Last modified:February 15, 2005 - v1
Checksum:i6B11099ED0AFB561
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38149.1.
RefSeqiWP_000417369.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38149; AAW38149; SACOL1320.
KEGGisac:SACOL1320.
PATRICi19528871. VBIStaAur112458_1288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA. Translation: AAW38149.1.
RefSeqiWP_000417369.1. NC_002951.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3G25X-ray1.90A/B/C/D1-498[»]
3GE1X-ray2.70A/B/C/D1-498[»]
ProteinModelPortaliQ5HGD2.
SMRiQ5HGD2. Positions 1-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL1320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW38149; AAW38149; SACOL1320.
KEGGisac:SACOL1320.
PATRICi19528871. VBIStaAur112458_1288.

Phylogenomic databases

eggNOGiENOG4108HMR. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiWWLTGGP.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciSAUR93062:GCEP-1298-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5HGD2.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin. 1 hit.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPK_STAAC
AccessioniPrimary (citable) accession number: Q5HGD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 15, 2005
Last modified: September 7, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.