Reviewed, UniProtKB/Swiss-Prot Q5HFZ7 (DYR_STAAC)

Names and originHide

Protein names

Recommended name:
Dihydrofolate reductase
Short name=DHFR
EC=1.5.1.3

Gene names

Name:	folA
Ordered Locus Names:	SACOL1461

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributesHide

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)Hide

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

OntologiesHide

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 159	158	Dihydrofolate reductase		PRO_0000186406
Regions
	Domain	2 – 157	156	DHFR
	Nucleotide binding	7 – 8	2	NADP By similarity
	Nucleotide binding	15 – 20	6	NADP By similarity
	Nucleotide binding	44 – 47	4	NADP By similarity
	Nucleotide binding	63 – 66	4	NADP By similarity
	Nucleotide binding	93 – 98	6	NADP By similarity
	Region	6 – 8	3	Substrate binding By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	Q5HFZ7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 811898409FEAFAAB	FASTA	159	18,251
	10 20 30 40 50 60 MTLSILVAHD LQRVIGFENQ LPWHLPNDLK HVKKLSTGHT LVMGRKTFES IGKPLPNRRN 70 80 90 100 110 120 VVLTSDTSFN VEGVDVIHSI EDIYQLPGHV FIFGGQTLFE EMIDKVDDMY ITVIEGKFRG 130 140 150 DTFFPPYTFE DWEVASSVEG KLDEKNTIPH TFLHLIRKK

ReferencesHide

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW36662.1.
RefSeq	YP_186310.1.
3D structure databases
SMR	Q5HFZ7. Positions 1-159.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HFZ7.
Genome annotation databases
EnsemblBacteria	EBSTAT00000008807; EBSTAP00000008542; EBSTAG00000008806.
GeneID	3238319.
GenomeReviews	Gene locus SACOL1461 in contig CP000046_GR.
KEGG	sac:SACOL1461.
TIGR	SACOL1461.
Phylogenomic databases
eggNOG	COG0262.
HOGENOM	HBG665708.
OMA	QDEEGVI.
ProtClustDB	CLSK885344.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL1461-MONOMER.
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	Q5HFZ7.

Entry informationHide

Entry name

DYR_STAAC

Accession

Primary (citable) accession number: Q5HFZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	January 23, 2007
Last modified:	July 13, 2010
This is version 44 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)