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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Staphylococcus aureus (strain COL)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60ShikimateUniRule annotation1
Active sitei64Proton acceptorUniRule annotation1
Binding sitei76NADPUniRule annotation1
Binding sitei85ShikimateUniRule annotation1
Binding sitei100ShikimateUniRule annotation1
Binding sitei209NADP; via carbonyl oxygenUniRule annotation1
Binding sitei211ShikimateUniRule annotation1
Binding sitei232NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 128NADPUniRule annotation5
Nucleotide bindingi148 – 153NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:SACOL1652
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000530 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360301 – 268Shikimate dehydrogenase (NADP(+))Add BLAST268

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi93062.SACOL1652

Structurei

3D structure databases

ProteinModelPortaliQ5HFG5
SMRiQ5HFG5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni13 – 15Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiFGNPIKH

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5HFG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFAVIGNPI SHSLSPVMHR ANFNSLGLDD TYEALNIPIE DFHLIKEIIS
60 70 80 90 100
KKELEGFNIT IPHKERIIPY LDYVDEQAIN AGAVNTVLIK DGKWIGYNTD
110 120 130 140 150
GIGYVKGLHS VYPDLENAYI LILGAGGASK GIAYELAKFV KPKLTVANRT
160 170 180 190 200
MARFESWNLN INQISLADAE KYLAEFDIVI NTTPAGMAGN NESIINLKHL
210 220 230 240 250
SPNTLMSDIV YIPYKTPILE EAERKGNHIY NGLDMFVYQG AESFKIWTNK
260
DADINSMKTA VLQQLKGE
Length:268
Mass (Da):29,879
Last modified:February 15, 2005 - v1
Checksum:i700864CB11686B89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000046 Genomic DNA Translation: AAW38268.1
RefSeqiWP_000666761.1, NC_002951.2

Genome annotation databases

EnsemblBacteriaiAAW38268; AAW38268; SACOL1652
KEGGisac:SACOL1652

Similar proteinsi

Entry informationi

Entry nameiAROE_STAAC
AccessioniPrimary (citable) accession number: Q5HFG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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