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Q5HF16 (SYL_STAAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SACOL1808
OrganismStaphylococcus aureus (strain COL) [Complete proteome] [HAMAP]
Taxonomic identifier93062 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152082

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif577 – 5815"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5801ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5HF16 [UniParc].

Last modified February 15, 2005. Version 1.
Checksum: CCEF7840099E907F

FASTA80591,786
        10         20         30         40         50         60 
MLNYNHNQIE KKWQDYWDEN KTFKTNDNLG QKKFYALDMF PYPSGAGLHV GHPEGYTATD 

        70         80         90        100        110        120 
IISRYKRMQG YNVLHPMGWD AFGLPAEQYA LDTGNDPREF TKKNIQTFKR QIKELGFSYD 

       130        140        150        160        170        180 
WDREVNTTDP EYYKWTQWIF IQLYNKGLAY VDEVAVNWCP ALGTVLSNEE VIDGVSERGG 

       190        200        210        220        230        240 
HPVYRKPMKQ WVLKITEYAD QLLADLDDLD WPESLKDMQR NWIGRSEGAK VSFDVDNTEG 

       250        260        270        280        290        300 
KVEVFTTRPD TIYGASFLVL SPEHALVNSI TTDEYKEKVK AYQTEASKKS DLERTDLAKD 

       310        320        330        340        350        360 
KSGVFTGAYA TNPLSGEKVQ IWIADYVLST YGTGAIMAVP AHDDRDYEFA KKFDLPIIEV 

       370        380        390        400        410        420 
IEGGNVEEAA YTGEGKHINS GELDGLENEA AITKAIQLLE QKGAGEKKVN YKLRDWLFSR 

       430        440        450        460        470        480 
QRYWGEPIPV IHWEDGTMTT VPEEELPLLL PETDEIKPSG TGESPLANID SFVNVVDEKT 

       490        500        510        520        530        540 
GMKGRRETNT MPQWAGSCWY YLRYIDPKNE NMLADPEKLK HWLPVDLYIG GVEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKVLY DLAIVPTKEP FQKLFNQGMI LGEGNEKMSK SKGNVINPDD IVQSHGADTL 

       610        620        630        640        650        660 
RLYEMFMGPL DAAIAWSEKG LDGSRRFLDR VWRLMVNEDG TLSSKIVTTN NKSLDKVYNQ 

       670        680        690        700        710        720 
TVKKVTEDFE TLGFNTAISQ LMVFINECYK VDEVYKPYIE GFVKMLAPIA PHIGEELWSK 

       730        740        750        760        770        780 
LGHEESITYQ PWPTYDEALL VDDEVEIVVQ VNGKLRAKIK IAKDTSKEEM QEIALSNDNV 

       790        800 
KASIEGKDIM KVIAVPQKLV NIVAK 

« Hide

References

[1]"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. expand/collapse author list , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: COL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000046 Genomic DNA. Translation: AAW38336.1.
RefSeqYP_186641.1. NC_002951.2.

3D structure databases

ProteinModelPortalQ5HF16.
SMRQ5HF16. Positions 1-803.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING93062.SACOL1808.

Proteomic databases

PRIDEQ5HF16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAW38336; AAW38336; SACOL1808.
GeneID3236033.
KEGGsac:SACOL1808.
PATRIC19529823. VBIStaAur112458_1767.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSAUR93062:GCEP-1769-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STAAC
AccessionPrimary (citable) accession number: Q5HF16
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: February 15, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries