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Q5HEM4

- DAGK_STAAC

UniProt

Q5HEM4 - DAGK_STAAC

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Protein

Diacylglycerol kinase

Gene
dagK, SACOL1958
Organism
Staphylococcus aureus (strain COL)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP By similarity
Binding sitei94 – 941ATP By similarity
Metal bindingi213 – 2131Magnesium; via carbonyl oxygen By similarity
Metal bindingi216 – 2161Magnesium By similarity
Metal bindingi218 – 2181Magnesium; via carbonyl oxygen By similarity
Active sitei273 – 2731Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATP By similarity
Nucleotide bindingi67 – 737ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. diacylglycerol kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR93062:GCEP-1953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:SACOL1958
OrganismiStaphylococcus aureus (strain COL)
Taxonomic identifieri93062 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000530: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 315315Diacylglycerol kinasePRO_0000386491Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi93062.SACOL1958.

Structurei

3D structure databases

ProteinModelPortaliQ5HEM4.
SMRiQ5HEM4. Positions 1-312.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKcAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiYLKGMEM.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5HEM4-1 [UniParc]FASTAAdd to Basket

« Hide

MRKRARIIYN PTSGKELFKR ELPDALIKLE KAGYETSAYA TEKIGDATLE    50
AERAMHENYD VLIAAGGDGT LNEVVNGIAE KPNRPKLGVI PMGTVNDFGR 100
ALHIPNDIMG ALDVIIEGHS TKVDIGKMNN RYFINLAAGG QLTQVSYETP 150
SKLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDGNVFQGE ALLFFLGLTN 200
SMAGFEKLVP DAKLDDGYFT LIIVEKSNLA ELGHIMTLAS RGEHTKHPKV 250
IYEKAKAINI SSFTDLQLNV DGEYGGKLPA NFLNLERHID VFAPNDIVNE 300
ELINNDHVDD NLIEE 315
Length:315
Mass (Da):34,887
Last modified:February 15, 2005 - v1
Checksum:iC5CC706B5E3A0F00
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000046 Genomic DNA. Translation: AAW38399.1.
RefSeqiYP_186783.1. NC_002951.2.

Genome annotation databases

EnsemblBacteriaiAAW38399; AAW38399; SACOL1958.
GeneIDi3237497.
KEGGisac:SACOL1958.
PATRICi19530191. VBIStaAur112458_1912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000046 Genomic DNA. Translation: AAW38399.1 .
RefSeqi YP_186783.1. NC_002951.2.

3D structure databases

ProteinModelPortali Q5HEM4.
SMRi Q5HEM4. Positions 1-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 93062.SACOL1958.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW38399 ; AAW38399 ; SACOL1958 .
GeneIDi 3237497.
KEGGi sac:SACOL1958.
PATRICi 19530191. VBIStaAur112458_1912.

Phylogenomic databases

eggNOGi COG1597.
HOGENOMi HOG000261395.
KOi K07029.
OMAi YLKGMEM.
OrthoDBi EOG613098.

Enzyme and pathway databases

BioCyci SAUR93062:GCEP-1953-MONOMER.

Family and domain databases

Gene3Di 3.40.50.10330. 1 hit.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain."
    Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H.
    , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M.
    J. Bacteriol. 187:2426-2438(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: COL.

Entry informationi

Entry nameiDAGK_STAAC
AccessioniPrimary (citable) accession number: Q5HEM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: February 15, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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