Q5HEK9 (NADE_STAAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SACOL1974

Organism

Staphylococcus aureus (strain COL) [Complete proteome] [HAMAP]

Taxonomic identifier

93062 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152194
Regions
	Nucleotide binding	47 – 54	8	ATP By similarity
Sites
	Active site	49	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5HEK9 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: BA4A032FFEFFD4A2	FASTA	273	30,697
	10 20 30 40 50 60 MSKLQDVIVQ EMKVKKRIDS AEEIMELKQF IKNYVQSHSF IKSLVLGISG GQDSTLVGKL 70 80 90 100 110 120 VQMSVNELRE EGIDCTFIAV KLPYGVQKDA DEVEQALRFI EPDEIVTVNI KPAVDQSVQS 130 140 150 160 170 180 LKEAGIVLTD FQKGNEKARE RMKVQFSIAS NRQGIVVGTD HSAENITGFY TKYGDGAADI 190 200 210 220 230 240 APIFGLNKRQ GRQLLAYLGA PKELYEKTPT ADLEDDKPQL PDEDALGVTY EAIDNYLEGK 250 260 270 PVTPEEQKVI ENHYIRNAHK RELAYTRYTW PKS

References

[1]

"Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain." Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J., Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J., Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H., Vamathevan J.J., Khouri H. , Utterback T.R., Lee C., Dimitrov G., Jiang L., Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E., Fraser C.M. J. Bacteriol. 187:2426-2438(2005) [PubMed: 15774886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: COL.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000046 Genomic DNA. Translation: AAW36944.1.
RefSeq	YP_186798.1. NC_002951.2.
3D structure databases
ProteinModelPortal	Q5HEK9.
SMR	Q5HEK9. Positions 4-268.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5HEK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000010627; EBSTAP00000010362; EBSTAG00000010626.
GeneID	3238369.
GenomeReviews	Gene locus SACOL1974 in contig CP000046_GR.
KEGG	sac:SACOL1974.
PATRIC	19530223. VBIStaAur112458_1928.
TIGR	SACOL1974.
Phylogenomic databases
eggNOG	COG0171.
GeneTree	EBGT00050000025036.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SAUR93062:SACOL1974-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STAAC

Accession

Primary (citable) accession number: Q5HEK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program